A BIOREACTOR FOR TWO-STAGE MULTIPLE SHOOT CULTURE OF CATHARANTHUS ROSEUS  

作　　者：元英进 胡宗定 

出　　处：《Chinese Journal of Chemical Engineering》1994年第2期33-38,共6页中国化学工程学报（英文版）

摘　　要：A self made gas-liquid jet bioreactor in spray flow mode is used in the two-stage multiple shoot culture of C. roseus. The experimental results show that the gas-liquid jet bioreactor is suitable for the multiple shoot culture of C. roseus in two stages, and the multiple shoots of C. roseus reach as high as 35g(dry wt.) · L-1 of biomass content and as high as 1.18% (dry wt.) of total indole alkaloids.1 INTRODUCTIONThe production of commercially important indole alkaloid from tissue culture of C.roseus hasbeen a major objective of research carried out worldwide[1].Of primary importance has beenthe production of catharanthine and vindoline which can be used as precursor for thesynthesis of commercially important antineoplastic agents,vinblastine and vincristine.

关 键 词：gas-liquid jet BIOREACTOR CATHARANTHUS roseus MULTIPLE SHOOTS TWO-STAGE culture indole alkaloids 

分 类 号：TQ021[化学工程]