恶性肿瘤患者外周血CD4^＋ CD25^high Foxp^3＋调节性T细胞的检测及临床意义  

Measurement of CD4^+ CD25^(high) Foxp3^+ regulatory T cells in peripheral blood of patients with cancer and clinical significance

作　　者：王雪野[1] 韩梅[1] 高长斌[2] 肖中平[1] 陈琳[1] 高鹏[1] 孔令环[1] 

机构地区：[1]吉林省人民医院血液风湿科,吉林长春130021 [2]吉林大学中日联谊医院干部科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2009年第2期373-376,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助课题(20070929);吉林省人事厅基金资助课题(2007)

摘　　要：目的:研究CD4+ CD25high Foxp3+调节性T细胞(Tregs)在恶性肿瘤患者外周血单个核细胞(PBMC)中的表达,探讨其在肿瘤发病中的作用及临床意义。方法:采用流式细胞术检测61例不同分期恶性肿瘤患者PBMC中CD4+ CD25+/CD4+、CD4+ CD25high/CD4+T细胞百分比,进一步分析CD4+ CD25+及CD4+ CD25high T细胞中表达Foxp3+ T细胞的百分比,并与正常对照组(n=32)进行比较。结果:与正常对照组比较,Ⅰ及Ⅱ期恶性肿瘤患者PBMC中CD4+ CD25+/CD4+及CD4+ CD25high/CD4+ T细胞百分比均无明显变化(P>0.05),Ⅲ及Ⅳ期恶性肿瘤患者均明显增高(P<0.05),随肿瘤恶性程度增高,CD4+ CD25+/CD4+及CD4+ CD25high/CD4+ T细胞的百分比也逐渐增高;恶性肿瘤患者CD4+ CD25+及CD4+ CD25high Foxp3+T细胞表达水平较正常对照组均增高,组间比较差异均有显著性(P<0.01)。结论:恶性肿瘤患者外周血调节性T细胞增高可能是恶性肿瘤发病及免疫机制异常的主要原因之一,定期检测外周血中上述指标的变化,有利于了解恶性肿瘤患者的病情变化及预后判断。Objective To study the expression of CD4^＋ CD25^high Foxp3^＋ regulatory T cells （Tregs） in peripheral blood monouclear cell （PBMC） of patients with cancer, and explore the significance of Tregs in pathogenesis of cancer. Methods The proportions of CD4^＋ CD25^＋/ CD4^＋ , CD4^＋ CD25^high/ CD4^＋ T cells in PBMC were examined with flow cytometry in 61 patients with cancer at different stages, and the expression levels of Foxp3^＋ in CD4^＋ CD25^＋ and CD4^＋ CD25^high T cells were analyzed, and compared with 32 normal control samples. Results Compared with normal controls, the proportions of CD4^＋ CD25^＋ / CD4^＋ , CD4^＋ CD25^high/ CD4^＋ T cells in PBMC of patients with cancer at stage I and stage Ⅱ had no significant difference （P〉0.05） ; but in those patients at stage Ⅲ and stage Ⅳ, they were higher than those in normal controls, the difference was significant （P〈0.05）. The proportions of CD4^＋ CD25^＋ / CD4^＋, CD4^＋ CD25^high/ CD4^＋ T cells were higher in PBMC of patients with cancer with higher histophathological grade. The expression levels of Foxp3^＋ in CD4^＋ CD25^＋ and CD4^＋ CD25^high T cells in patients with cancer were also higher than those in normal controls （P〈0.01）, the differences were significant （P〈0.01）. Conclusion The increase of Tregs in patients with cancer may be related to tumor progression and it shows immunological abnormality in patients with cancer. The regular detection of CD4^＋ CD25^＋ , CD4^＋ CD25^high and CD4^＋ CD25^high Foxp3^＋ in PBMC will contribute to understanding the patient＇s condition and judging prognosis.

关 键 词：恶性肿瘤 调节性T细胞 发病 预后 

分 类 号：R730.45[医药卫生—肿瘤]