人精浆SDS-聚丙烯酰胺梯度凝胶电泳谱型研究  

ON THE PROTEIN BAND PATTERNS OF THE HUMAN SEMINAL PLASMA USING SDS-POLYACRYLAMIDE GRADIENT GEL ELECTROPHORESIS(SDS-PAGGE)

作　　者：铁坚[1] 贾静涛[1] 

机构地区：[1]中国医科大学法医学系法医血清学教研室

出　　处：《中国法医学杂志》1990年第4期209-213,261,共6页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金

摘　　要：本文采用SDS-聚丙烯酰胺梯度凝胶电泳(SDS-PAGGE)研究了102例中国正常男子精浆蛋白的电泳谱型,得到了具有精液特征性的,可与人阴道液、唾液、初乳及血清进行鉴别的人精浆蛋白电泳谱型,并可作为精斑的确证。另发现一种-83kd 变异型。频率为8. 82±2. 81%。提高了精斑的个人识别机率,在实际应用中取得了初步成效。The protein band patterns of 102 Chinese male's seminal plasma demonstrated bySDS-PAGGE were analyzed.The common electrophoretic patterns consist of 40 ormore protein bands belonging to 3 divisions.Some band patterns are uniqus for theseminal plasma so that the seminal plasma can be distinguised from the human va-ginal fluid,saliva,colostrum and serum In addition,a variant band called 83kdtentatively was discovered in the seminal plasma.Its frequency is 8. 22±2. 81%.TheSDS-PAGGE pattern was successfully applied to the case work.

关 键 词：人精浆 SDS-PAGGE 电泳谱型 变异型 

分 类 号：D919[医药卫生—法医学]