烟草薄层培养器官发生的控制及细胞学观察  

CONTROL OF MORPHOGENESIS AND ITS CYTOLOGICAL STUDY IN EXCISED THIN-LAYER CULTURES OF FLOWERING NICOTIAN A TABACUM EXPLANTS

作　　者：章美云[1] 韩碧文[1] 

机构地区：[1]北京农业大学

出　　处：《植物学报》1989年第2期86-89,共4页Chinese Bulletin of Botany

摘　　要：普通烟草(Nicotiana tabacum)花梗表皮薄层组织在不同生长素和细胞分裂素配比的MS培养基上及不同的培养条件下,可分别诱导,得到直接发生的营养芽和花芽,以及根和不发生器官分化的愈伤组织。组织间的相互联系,影响器官发育潜能的发挥。细胞学观察发现,直接发生的营养芽和花芽起源于薄层组织的亚表皮细胞层。De novo differentiation of direct vegetative and floral buds, roots and nonorganogenetic callus can be obtained from excised thin cell layers (one to six layers of epidermal and subepidermal cells) of flower stalk sections of N. tabacum L.c.v. Samsun by varying the auxin-cytokinin ratio in MS' medium and cultural conditions: Inter-tissue correlations affect the organogenetic capacities of various tissues of the stem segments. Cytological study shows that the vegetative and floral buds originated directly from the subepidermal layers.

关 键 词：薄层培养 器官发生 烟草 

分 类 号：Q94[生物学—植物学]