Der p2重组BCG对哮喘小鼠气道炎症的抑制作用  

Suppression of airway inflammation in a mouse model of asthma by Der p2 recombined BCG

作　　者：欧阳海峰[1] 史皆然[1] 刘阿茹[1] 遆新雨[1] 宋立强[1] 吴昌归[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033

出　　处：《第四军医大学学报》2009年第7期599-602,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金面上项目(30570804)

摘　　要：目的:观察胞壁型屋尘螨抗原Derp2重组卡介苗(Derp2rBCG)对哮喘小鼠气道炎症的抑制作用.方法:实验分4组,小鼠分别给予生理盐水、BCG或Derp2rBCG处理,然后以Derp2为变应原建立哮喘模型,正常对照组给予生理盐水致敏及气道内滴入,取肺泡灌洗液、肺组织标本,观察肺部炎症细胞浸润、气道杯状细胞增殖及肥大细胞脱颗粒情况.结果:①Derp2rBCG下调了哮喘小鼠气道局部炎症;②Derp2rBCG减轻了哮喘小鼠肺组织中的炎症细胞浸润;③Derp2rBCG可以减轻哮喘小鼠气道粘液高分泌现象并下调杯状细胞数量;④Derp2rBCG减轻了哮喘小鼠气道中的肥大细胞脱颗粒现象.结论:Derp2rBCG是一种有效的哮喘免疫治疗方法.AIM： To observe whether Der p2 rBCG suppress ES the airway inflammation in a mouse model of asthma. METHODS ： Four groups of mice were vaccinated with PS, BCG or rBCG and were sensitized and challenged with Der p2. Bronchoalveolar lavage fluid and lung tissue were then obtained. The infiltration of inflammatory cells, goblet cell proliferation and mast cell degranulation were observed. RESULTS： Airway inflammation was downregulated by Der p2 rBCG in a mouse model of asthma. The infiltration of inflammatory cells in lung tissue was downregulated by Der p2 rBCG. The production of mucus and the number of goblet cells was decreased by Der p2 rBCG. Mast cell mediator release was reduced by Der p2 rBCG. CONCLUSION： Der p2 rBCG may be an efficient immunotherapy for asthma.

关 键 词：卡介苗 尘螨蛋白2组 哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]