极早熟葡萄胚珠培养技术的研究  

STUDY ON TECHNIQUES OF IN-OVULO EMBRYO CULTURE OF VERY EARLY-RIPENING GRAPE

作　　者：贺普超[1] 董晓玲[1] 

机构地区：[1]西北农业大学

出　　处：《果树学报》1989年第4期199-204,共6页Journal of Fruit Science

摘　　要：简化极早熟葡萄胚胎培养的操作,提高萌发成苗率及培养健壮幼苗技术的研究结果表明:胚珠横切半处理不仅可以提高胚的萌发成苗率,促使试管实生苗健壮生长,而且操作极简便;筛选出了胚珠内胚萌发成苗的最佳激素浓度组合和最适培养基;胚龄和充分的低温处理也是提高胚萌发成苗率,培养健壮幼苗的两大关键因素。In this experiment, the techniques of simplying the operation forin-ovulo embryo culture of very early-ripening grape, raising the percent of germination and seedling production, culturing vigorous seedling were studied. The results were as followings the treatment that ovule were crossed cut in half not only can obtain high percent of germination and seedling production, and seedling grow vigorous, but also can simply the operation for in-ovulo embryo culturing of grape. The best hormone combination and best medium for in-ovulo embryo culturing were have been selected. We also found that embryo age and enough low temperature treatment were two key factors for raising the percent of germination and seedling production of in-ovulo embryo and culturing vigorous seedling.

关 键 词：胚珠培养 葡萄品种 最佳激素 成苗率 低温处理 浓度组合 离体胚培养 试管苗 离体培养 花后 

分 类 号：S66[农业科学—果树学]