人死亡后特定条件下骨髓细胞核DNA降解与死后时间间隔推断  

Relationship between DNA degradation in human marrow nuclei and late postmortem interval after death

作　　者：罗光华[1] 靳俊峰[2] 高翠莲[3] 王江峰[3] 陈玉川[3] 

机构地区：[1]广东司法警官职业学院司法鉴定系,广东广州510520 [2]遵义医学院珠海校区,广东珠海519041 [3]广东警官学院,广东广州510232

出　　处：《第四军医大学学报》2009年第7期661-663,共3页Journal of the Fourth Military Medical University

基　　金：广东省自然科学基金(036628)

摘　　要：目的:探讨人死亡后骨髓细胞核DNA含量变化与晚期死后时间间隔关系.方法:离体人胸骨置于特定条件下,随时间延长分别取材制成病理切片,应用DNA高度特异性染色方法Feulgen改良法结合计算机图像分析技术对死后不同时间胸骨骨髓细胞核DNA含量变化进行分析.结果:人死亡后1～10d,Feulgen染色阳性产物随死亡时间延长逐渐减少,胸骨骨髓细胞核DNA含量随死亡时间延长呈规律下降,DNA染色逐渐变浅淡,至死后10d已基本观察不到.结论:人死亡后骨髓细胞核DNA含量随死亡时间延长呈下降趋势,两者之间存在线性关系.AIM： To study the relationship between degradation of DNA in human marrow nuclei and estimation of postmortem interval. METHODS ： The amount of nuclear DNA in the marrow of sternum was determined from 1 to 10 d after death stained by improved Feulgen histochemical method with image analysis. RESULTS ： The content of marrow DNA decreased gradually with the prolongation of postmortem interval and gradated to its minimum after 10 d after death. CONCLUSION： The content of nuclear DNA in human marrow of sternum reduces with extending time and there is a linear relationship between the degradation rate of the nuclear DNA and postmortem interval.

关 键 词：死后时间间隔 DNA含量 腐败尸体 胸骨骨髓 

分 类 号：R503[医药卫生—内科学]