红细胞吸附解脱提纯兔瘟病毒  

PURIFICATION OF RABBIT PEST VIRUS BY RED BLOOD CELL ABSORPTION AND DESORPTION

作　　者：阎秉芳[1] 

机构地区：[1]华中农业大学牧医系单克隆研究室

出　　处：《畜牧兽医学报》1989年第S1期111-113,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：高速匀浆兔瘟病兔肝、脾、肾等,制备组织悬液。将上清用人“O”型红细胞吸附,再调节pH9.2,使之病毒解脱,从而获得高纯度的病毒粒子。结果表明,该方法简便、高效、费时少、耗资低。The tissue suspension was made with liver, spleen and kidney of rabbits died of rabbit post using high speed pounding. The supernatant iluid was treated with 'O' type red blood cell ( RBC ) of human being so that RBC absorbed the virus in the fluid after washing the RBC three times using normal physiological saline, the virus was free from RBC by adjusting the pH in the red blood cell suspension to 9.2. The results showed that the method was simple, effective, saving time and low cost.

关 键 词：兔瘟 细胞吸附 兔肝 匀浆机 上清 兽医界 吸附活性 滴度 临床诊断 组织匀浆 

分 类 号：S8[农业科学—畜牧兽医]