用牛SL和SD抗原的PPA-ELISA检测人班氏丝虫病抗体的研究  

APPLICATION OF PPA-ELISA FOR DETECTING THE ANTIBODY OF HUMAN BANCROFTIAN FILARIASIS USING TWO ANTIGENS S.L. & S. D.

作　　者：徐礼周[1] 叶萍[1] 钟志萍[1] 

机构地区：[1]中国农业科学院上海家畜血吸虫病研究所

出　　处：《畜牧兽医学报》1989年第S1期132-136,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：牛唇乳突腹腔丝虫(Setaria labiatopapilosa,称S.L.)和牛指状腹腔线虫(setarta digi-tata,称S.D.)各自被制备的抗原,经酶标记葡萄球菌A蛋白的酶联免疫吸附试验(PPA-ELISA),对福建丝虫病流行区已知不同感染度微丝蚴的46例班氏丝虫病人血清检测结果:S.L.抗原的阳性检出率为95％(23/24和44/46),S.D.抗原阳性检出率为88％(21/24),而且S.L.抗原的光密度值(OD值)较普遍高于S.D.抗原。对照组健康人血清和囊虫病人血清各8例均无交叉反应,其OD值均在阴性幅度内。Both antigen of Selaria labiatopapillosa ( S. L. ) and Setaria digitata ( S. D. ) from the abdominal cavity of cattle had been prepared and used in ELISA of HRP-Protein A ( PPA-ELISA ) . 46 sera of the patients affected with filariasis were collected from filaria opidemic area in Fu Jian Province. Positive results to antigen S. L. were obtained 95% and to the other ——antigen S, D. 88%, the optical density of the former was higher than that of the latter. Among the sera of 8 healthy control and 8 Cysticercosis cases there were no cross-reaction and the optical density was in the negative area. It is suggested that S. L. and S.D. might be served as a kind of satisfactory heterogeneous antigen and PPA-ELISA might be a useful laboratory method in the diagnosis of human bancroftian filariasis.

关 键 词：班氏丝虫病 PPA-ELISA检测 SD抗原 SL 抗原检测 微丝蚴 感染度 唇乳突 无交叉反应 酶标记 

分 类 号：S8[农业科学—畜牧兽医]