棉酚的不同旋光异构体抗生育作用的研究  

MECHANISM OF ANTIFERTILITY BY GOSSYPOL OPTICAL ISOMERS IN RATS

作　　者：卢清显 陈啸梅[1] 刘平[1] 程凯[1] 沈晓明 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《解剖学报》1989年第S1期37-42,112-113,共8页Acta Anatomica Sinica

摘　　要：本实验采用12种组织化学方法观察棉酚旋光异构体对灌喂(+),(±)棉酚每日30mg/kg及(-)棉酚每日15mg/kg的大鼠生殖系统的影响.结果表明:(+)棉酚无抗生育作用.(±),(-)棉酚均能引起大鼠精于发生过程出现动力学紊乱,抑制晚期精子细胞核蛋白的替代与转换,使附睾尾精子头、尾断裂、线粒体酶反应颗粒变粗,数量减少,酶活性受抑制。但是,反映睾丸间质细胞雄激素合成功能的G6PDH和3β-HSDH以及表明附睾雄激素释放功能的ALP均未改变,说明棉酚对雄激素合成与分泌无影响。12 kinds of histochemical methods were adopted to evaluate the effects of gossypol optical isomers on the reproductive system of Wistar rats, following the administration of (±) and (+)-gossypol at a dosage of 30mg/kg daily, and(-)-gossypol at a dosage of 15 mg/kg daily, respectively for 2 or 3 weeks. (+)-gossypol has no an tifertility activity. (±) and (-)-gossypol caused kinetic disorder of spermatogemsis, and inhibited turnover of nuclear basic protein of the late, elongated spermatids in the testis. No changes in G6PDH and 30-HSDH histochmical reaction in Leydig cells, and ALP in the basement membrane of epididymis were observable reflecting that gossypol has no effect on synthesis and secretion of testosterone. Spermatozoa in the epididymis of rats treated with (±) and (-)-gossypol exhibited head and tail separation. The formazan deposits of reaction of LDH, LDH-X, MDH and ICDH located in sperm mitochondria appeared to become coarse and to reduce in quantity after (±) and (-)-gossypol treatment.

关 键 词：棉酚旋光异构体 抗生育作用 组织化学 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]