华蟾素对卵巢癌3AO细胞存活素表达的影响和意义  

Effect and significance of cinobufacine on the expression of survivin in the ovarian carcinoma 3AO cells

作　　者：王绍光[1] 姜学强[1] 

机构地区：[1]烟台市毓璜顶医院妇科,山东省烟台264000

出　　处：《中华航海医学与高气压医学杂志》2009年第1期45-47,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：山东省烟台市科研项目（2005126）

摘　　要：目的探讨华蟾素对体外培养的卵巢癌3AO细胞存活素表达的影响和意义。方法体外培养卵巢癌3AO细胞，采用不同浓度的华蟾素干预后，应用RT—PCR检测存活素的表达，流式细胞仪检测细胞的凋亡。结果卵巢癌3AO细胞对照组的存活素mRNA表达为1．01±0．13，细胞凋亡率为（2．31±0．98）％；0．25g／L华蟾素对卵巢癌3AO细胞存活素mRNA和细胞凋亡率无影响（P〉0．05）；2．5g／L华蟾素干预后，卵巢癌3AO细胞存活素mRNA表达为0．67±0．16，癌细胞凋亡率为（28．69±4．16）％，与对照组相比差异均有统计学意义（P〈0．05）；25g／L华蟾素与2．5g／L华蟾素对卵巢癌3AO细胞存活素mRNA和癌细胞凋亡率的影响差异无统计学意义（P〉0．05）。结论2．5g/L华蟾素即可抑制卵巢癌3AO细胞存活素mRNA的表达，抑制癌细胞的增殖。Objective To investigate the effect and significance of cinobufacine on the expression of survivin in the ovarian carcinoma 3AO cells cultured in vitro. Methods Ovarian carcinoma 3AO cells were cultured in vitro Following intervention of cinobufacine with different concentrations, the expression of survivin was measured by RT-PCR, and cell apoptosis was tested by flow cytometry. Results For the control group, the expression of survivin mRNA in the ovarian carcinoma 3AO cells was 1. 01 ± 0. 13, cell apoptotic rate was （2. 31 ± 0.98）%. Nevertheless, 0. 25 mg/ml cinobufacine didn＇t affect survivin and cellular apoptotic rate （ P 〉 0. 05 ）. Following administration of 0.25 mg/ml cinobufacine, the expression of survivin mRNA was 0.67 ± 0.16, and cell apoptotic rate was （28.69 ± 4.16）%. Statistical differences could be seen when it was compared with that of ovarian carcinoma 3AO cells in the control group（ P 〈 0. 05 ）. There were no statistical differences in the effect that survivin mRNA expression and cell apoptotie rate were affected following applications of either 25 mg/ml or 2.5 mg cinobufacine （ P 〉 0.05 ）. Conclusions 2.5 mg/ml cinobufacine could inhibit the expression of ovarian carcinoma 3AO ceils.

关 键 词：华蟾素 卵巢癌 3AO细胞 存活素 凋亡 

分 类 号：R686[医药卫生—骨科学]