自生固氮菌第二固氮酶的研究——Ⅰ.菌种的分离和鉴定  

STUDIES ON THE SECOND NITROGENASE OF AZOTOBACTER 1. THE SELECTION AND IDENTIFICATION OF STRAIN

作　　者：戴祥鹏 靳素英[1] 吕群燕 段俊英[1] 杨俊森[1] 刘筱春 

机构地区：[1]中国科学院沈阳应用生态研究所

出　　处：《微生物学杂志》1989年第2期35-36,34,共3页Journal of Microbiology

基　　金：国家自然科学基金;中国科学院重点项目

摘　　要：从四川攀枝花钒铁矿周围的土壤中分离出一株具有较高乙炔还原活性的固氮菌,经鉴定为棕色固氮菌(Azotobacter vinelandii),在Asby's培养基上生长时产生黄绿色荧光、还原乙炔。当培养基中加入1μM NaVO_3时,生长旺盛,产生深绿色荧光,具有乙炔还原活性。实验表明,该菌株含有第二固氮酶。A strain of diazotroph which can reduce C_2H_2 in a higher rate has been isolated from the soil near the Pan Zhi Hua vanadiumiron mine at Dukou city in Sichuan province. It has been characterized to be a strain of Azotobacter vinelandii. It grows well on Asby's medium and produces green-yellow fluorescence. When 1μmol NaVO_3·2H_2O is added it grows much better, producing a dark-green fluorescence and reduces C_2H_2. This indicates that the strain of Azotobacter vinelandii posseses the second nitrogenase which utilizes vanadium.

关 键 词：自生固氮菌 固氮酶 还原活性 黄绿色荧光 四川攀枝花 钒铁 甲基红试验 碳源利用 荧光色素 深绿色 

分 类 号：Q93[生物学—微生物学]