温度变化对δ-氨基酮戊酸诱导的Hep-2细胞光动力学效应的影响  

Effects of elevating temperature on photodynamic reaction in laryngeal squamous carcinoma cells induced by δ-aminolevullnic acid

作　　者：张毅[1,2] 夏育民[1] 刘小明[1] 车巍[1] 熊腊元[1] 徐世正[1] 

机构地区：[1]武汉大学人民医院皮肤科,430060 [2]三峡大学第三临床学院葛洲坝中心医院内二科

出　　处：《中华医学杂志》2009年第14期994-996,共3页National Medical Journal of China

摘　　要：目的探讨温度变化对δ-氨基酮戊酸（AIJA）诱导的喉鳞癌系Hep-2细胞光动力学效应的影响。方法将Hep-2细胞分成实验组（含2mmol／LAIJA）和对照组（未加ALA），在19～46℃时避光孵育。经激光照射诱导光动力反应之前，检测胞内原卟啉Ⅸ（PpⅨ）水平和细胞死亡率（坏死与凋亡）；激光照射后，再次检测细胞死亡率。结果照光前随着温度上升，实验组胞内PpⅨ水平由（0．25±0．06）μg／L提高至（1．07±0．11）μg／L，对照组胞内未能测出PpⅨ。照光前，在19～37℃区间，两组细胞的死亡率变化不明显；在37～46℃区间，实验组细胞死亡率由2．33％增加至38．99％，对照组由1．79％增加至36．66％，两组间死亡率差异无统计学意义（P〉0．05）。照光后：对照组的细胞死亡率变化与照光前相同，而实验组则随着温度增加而升高（31．11％～98．92％）；在相同温度下实验组结果均高于对照组（P〈0．05），且随着温度上升，两组差值由28．99％增大至59．26％。结论适度升高培养温度可以增强ALA诱导的Hep-2细胞光动力学效应。Objective To investigate effect of temperature on 8-aminolevulinic acid （ALA） induced photodynamic reaction in laryngeal squamous carcinoma cells. Methods Human laryngeal squamous carcinoma cells of the line Hep-2 cells were co-cultured with 2 mmol/L ALA （ Group A） or without ALA （Group B） at the culturing temperature 19 -46℃. Three hours later cellular protoporphyrin IX （PpIX） level was determined by high performance liquid chromatography with fluorescent detection. Laser scanning confocal microscopy was used to observe the fluorescent strength in the Hep-2 cells. The ratio of cell death （including necrosis and apoptosis ） was detected with flow cytometer before and after phtotodynamic reaction. Results In Group A the Pp IX level at the temperature of 19℃ was （0.25± 0.06） μg/L, and raised to （ 1.07±0.11）μg/L at 46 ℃. There was no cellular PpIX detected in Group B at any temperature. Before laser radiation the cell death ratios of both groups were the same at the temperature 19-37 ℃, and at the temperature 37 - 46℃ . After laser radiation the cell death ratio of Group A raised from 31.11% to 98.92% as the temperature went up steadily, but the results of Group B showed the same curve as before laser radiation. At any same temperatures the cell death ratios of Group A were all significantly higher than those of Group B （ all P 〈 0.05 ）, and as the temperature was elevated the difference between the ：2 groups raised from 28. 99% to 59. 26%. Conclusion Moderate higher temperature enhances the Pp IX production and photodynamic reaction in human laryngeal squamous carcinoma induced by ALA in vitro.

关 键 词：氨基酮戊酸 光化学疗法 温度 血红素 HEP-2细胞 

分 类 号：R686[医药卫生—骨科学]