硝酸甘油和硝酸异山梨酯抗柯萨奇B组3型病毒感染的体内实验研究  

Effect of Glyceryl Trinatrate and Isosorbide Dinitrate Against Replication of Coxsackievirus B3 in vivo

作　　者：孙艳影[1] 杜凤霞[1] 夏美玲[2] 孙艳[1] 钟照华[3] 

机构地区：[1]齐齐哈尔医学院医学技术学院病原教研室,161006 [2]齐齐哈尔医学院医学技术学院检验中心 [3]哈尔滨医科大学微生物学研室

出　　处：《医学研究杂志》2009年第4期85-88,共4页Journal of Medical Research

摘　　要：目的研究硝酸甘油(glyceryl trinatrate,GTN)和硝酸异山梨酯(isosorbide dinitrate,ISDN)抗柯萨奇B组3型病毒(CVB3)的作用,为GTN和ISDN今后在抗CVB性心肌疾病的临床应用提供实验依据。方法用HeLa细胞扩增并收获CVB3后,测定CVB3的半数组织培养感染剂量(TCID50),BALB/c小鼠腹腔注射5000TCID50的CVB3,同时给予GTN和ISDN,14天后处死小鼠,取心脏做常规组织病理学检查。结果用GTN、ISDN保护的小鼠心肌病理标本每视野(×100)病灶数明显低于病毒对照组,GTN组为0.89±0.18,ISDN组为1.25±0.22,与病毒对照组差异显著(P<0.05)。结论GTN、ISDN等临床常用于抗心绞痛的硝酸酯类药物具有明确的抗CVB3感染BALB/c小鼠心肌细胞的作用。Objective To provide an experimental evidence for the clinical applications of glyceryl trinatrate （GTN） and isosorbide dinitrate （ISDN） against Coxsackicvirus B （CVB） - related myocarditis. Methods Coxsackievirus B3 was propagated in HeLa cells. Virus yields were determinded by 50% tissue culture infective dosage （TCID50）. BALB/c mice were attacked with 5000 TCID50 of CVB3, meanwhile, the mice were administrated with GTN and ISDN. All mice were killed at the 14th day. The myocardial tissues were harvested for histologic evaluation. Results The infection plaques in the myocardial tissues obtained from CVB3 - infected BALB/c mice treated with GTN were siginifieantly reduced （0.89±0.18 in GTN group and 1.25±0.22 in ISDN group） compared with that of the untreated CVB3 - infected mice （P 〈 0.05）. Conclusion GTN and ISDN, the vasodilator had widely used in the treatment of angina pectoris, exhibited definitely antiviral activity in CVB3 -infected BALB/c mice.

关 键 词：B组柯萨奇病毒 硝酸甘油 硝酸异山梨酯 一氧化氮 抗病毒 

分 类 号：R285.5[医药卫生—中药学] R541.405[医药卫生—中医学]