疫霉侵染的辣椒cDNA文库的构建及非特异性脂转移蛋白cDNA的筛选  

Construction for cDNA library of pepper infected by Phytophthora capsici.,and the screening of nsLTP cDNA from library

作　　者：林明[1] 贺俐[2,3] 徐波[1] 刘志钦[1] 王育娜[1] 官德义[2] 马洪丽[1] 何水林[1] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学作物科学学院,福建福州350002 [3]井冈山大学生命科学学院,江西吉安343009

出　　处：《福建农林大学学报（自然科学版）》2009年第2期150-153,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金(30571128;30600391);福建省自然科学基金(2008J0003)资助项目

摘　　要：建立了辣椒疫霉侵染的辣椒幼苗cDNA文库,该原始文库含有1.5×106个独立克隆,插入片段约为0.5-2 kb.采用基于PCR技术的96孔文库筛选方法,从该cDNA文库中筛选出了1个应答辣椒疫霉侵染的候选基因的全长cDNA.经生物信息学分析,推测这个候选基因为非特异性脂转移蛋白(non-specific lipid-transfer protein,nsLTP)基因.The total RNA was extracted from young pepper infected by Phytophthora capsici., and the cDNA library including more than 1.5 ×10^6 individual clones was constructed with SMARTTM cDNA construction kit. A positive candidate resistant gene cDNA was isolated by 96-hole screening method based on PCR. The results of bioinformatics analysis showed that the candidate nsLTP resistant gene could code the non-specific lipid-transfer protein.

关 键 词：辣椒 辣椒疫霉 CDNA文库 非特异性脂转移蛋白 

分 类 号：Q78[生物学—分子生物学]