鲁道夫对盲囊线虫C新种的ITS rDNA序列测定及种系发育关系分析  

ITS rDNA sequencing and phylogenetic analysis of the new species Contracaecum rudolphii C(Nematoda:Anisakidae)

作　　者：张媛[1,2] 林瑞庆[1] 赵光辉[1] 袁子国[1] 宋慧群[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2009年第4期298-302,共5页Chinese Veterinary Science

基　　金：家畜疫病病原生物学国家重点实验室(中国农业科学院兰州兽医研究所)开放基金课题;教育部“长江学者和创新团队发展计划”创新团队项目(IRT0723);广东省自然科学基金团队项目(05200638)

摘　　要：以鲁道夫对盲囊线虫C种为研究对象,对其rDNA的内转录间隔区(ITS)序列进行PCR扩增、测序和序列分析,以ClustalⅩ1.81程序对序列进行比对,然后用MEGA4.0程序的MP法和NJ法及PUZ-ZLE4.1程序的ML法构建鲁道夫对盲囊线虫A、B、C种以及北方对盲囊线虫的种系发育关系。结果显示,鲁道夫对盲囊线虫C种的ITS-1,5.8 S,ITS-2序列大小分别为451 bp、127 bp和277 bp,鲁道夫对盲囊线虫3个种与北方对盲囊线虫ITS序列种内差异小,种间差异明显。种系发育关系分析发现,这3种方法(NJ法、MP法和ML法)构建的种系发育树的拓扑结构基本一致,其中C种和A种聚在一起,构成一支,再和B种聚在一起;北方对盲囊线虫单独成一支。研究结果证明鲁道夫对盲囊线虫C种确为一新种。The internal transcribed spacers （ITS） of Contracaecum rudolphii C were amplified, sequenced and compared with those of C. rudolphii A,C. rudolphii B,and C. septentrionale available in Gen- Bank^TM, and followed by phylogenetic reconstruction. The sequences were aligned using the Clustal X 1.81 ,MP and NJ trees were reconstructed using the softwares MEGA version 4.0 ,and ML tree was reconstructed using PUZZLE version 4.1. The results revealed that the ITS sequences of C. rudolphii C was 855 bp in length,including 451 bp for ITS 1,127 bp for 5.8S and 277 bp for ITS 2,and that the average intraspecific variations were comparatively lower and the inter-specific differences were significantly higher. Topologies of 3 phylogenetic trees inferred by different methods（NJ, MP or ML） were identical or similar. These phylogenetic trees showed two distinct clades：C, rudolphii and C. septentrionale. In the C. rudolphii clade,C, rudolphii A was genetically closer to C. rudolphii C than to C. rudolphii B.

关 键 词：鲁道夫对盲囊线虫C种 内转录间隔区 RDNA 种系发育关系分析 

分 类 号：S852.731[农业科学—基础兽医学]