多孔明胶微球/磷酸钙骨水泥的制备及其性能研究  

Development of macroporous calcium phosphate cements as bone substitutes by using gelatin microspheres

作　　者：厉孟[1] 刘旭东[1] 刘兴炎[1] 葛宝丰[1] 

机构地区：[1]兰州军区兰州总医院骨科,兰州730050

出　　处：《中国矫形外科杂志》2009年第7期526-529,共4页Orthopedic Journal of China

基　　金：全军"十一五"医药卫生科研基金资助项目(编号:06MA090);甘肃省自然科学基金资助项目(编号:0710RJZA068)

摘　　要：[目的]制备多孔明胶微球/磷酸钙骨水泥,并于体内体外研究其各种性能。[方法]双相乳化冷凝聚合法制备明胶微球,以不同比例与磷酸钙骨水泥复合(0%,2.5%,5%),制备多孔磷酸钙骨水泥,测定材料孔径率及抗压强度,筛选出最佳比例。消化法培养成骨细胞接种于常规及多孔磷酸钙骨水泥支架上,扫描电镜观察细胞形态;不同材料浸提液(0%,2.5%明胶微球/磷酸钙骨水泥及聚苯乙烯)分别与成骨细胞共培养,以MTT法测定细胞增殖率,试剂盒检测碱性磷酸酶水平。将磷酸钙骨水泥及2.5%明胶微球/磷酸钙骨水泥分别植入山羊椎体内,6个月后收集标本,分别进行X线影像学及组织学观察,评估其降解情况。[结果]不同比例明胶微球/磷酸钙骨水泥的总孔径率、大孔率及抗压强度分别为:38.7%、0%、12.1MPa(0%);67.5%、40.6%、8.0MPa(2.5%);72.2%、45.6%、5.0MPa(5%)。成骨细胞在明胶微球/磷酸钙骨水泥上生长良好,细胞增殖及碱性磷酸酶水平均明显高于单纯磷酸钙骨水泥组,与聚苯乙烯组未见明显差异。多孔明胶微球/磷酸钙骨水泥6个月后在体内大部分已降解,而磷酸钙骨水泥未见明显降解。[结论]复合明胶微球可显著提高磷酸钙骨水泥的孔径率促进其降解,增加其生物活性,这种多孔磷酸钙骨水泥可作为非负重部位的骨替代物。[Objective]To develop macroporous calcium phosphate cements（CPCs） by incorporating the gelatin microspheres（GMs） and to evaluate the performance of the composite as a bone substitute in vitro and in vivo. [Methods]GMs were prepared by improved emulsified cold-condensation method and macroporous CPCs were developed using GMs with three different weight ratios （0%,2.5% and 5%）. The porosity and the compression strength of the three composites were measured to determine the proper weight ratio of GMs. The morphologic changes of osteoblasts were examined with scanning electron microscope at two days after the cells were seeded on the CPCs and GMs/CPCs. The cells were also cultured with different leaching liquor of three kinds of materials （0%,2.5% GMs/CPCs and polystyrene）.Cell viability analysis was performed by MTT assay,and alkaline phosphatase was measured with alkaline phosphatase kit. At six months after implanted in the goat vertebre ,the samples with CPCs or GMs/CPCs were obtained and evaluated by X-ray image and histological anatomy.[Results]Porosity of the GMs/CPCs increased with the increase of GMs,but compression strength decreased. The proliferation and differentiation of the osteoblasts were enhanced with the 2.5% GMs/CPCs compared to the CPCs. In vivo study showed that the degradation of GMs/CPCs was improved.[Conclusion]Macroporous calcium phosphate cements can be developed by incorporating gelatin microspheres,and biocompatibility and degradability of the composite can be enhanced. The composite can be used as a bone substitute under non-loaded circumstances.

关 键 词：多孔磷酸钙骨水泥 明胶微球 孔径率 抗压强度 骨替代物 

分 类 号：R318.08[医药卫生—生物医学工程]