仙灵骨葆对兔膝骨性关节炎治疗作用的研究  

Effect of XLGB treatment in the rabbit anterior cruciate ligament transaction models of osteoarthritis

作　　者：程潭[1] 张柳[1] 李震[1] 李强[1] 穆树林[1] 田发明[1] 刘永强[1] 

机构地区：[1]华北煤炭医学院骨外科,河北唐山063000

出　　处：《中国矫形外科杂志》2009年第7期530-533,共4页Orthopedic Journal of China

摘　　要：[目的]研究仙灵骨葆(xianlinggubao,XLGB)对兔骨性关节炎关节软骨及软骨下骨的保护作用。[方法]30只3个月龄雄性日本大耳白兔,随机分为对照组(Sham)、盐水组(ACLT+NS)和仙灵骨葆组(ACLT+XLGB),每组各10只。盐水组和仙灵骨葆组行右膝关节前交叉韧带切断术(anterior cruciate ligament transaction,ACLT)造成膝骨性关节炎模型。术后仙灵骨葆组125mg/(kg·d)灌胃;盐水组则给予等剂量生理盐水。6周后处死动物,取股骨测量骨密度,之后脱钙制片,行HE染色、Mankin评分及BMP-2、MMP-13免疫组化染色;取右膝胫骨近端包埋,制成硬组织切片,用于骨组织形态计量学测量。[结果](1)Mankin评分:仙灵骨葆组显著低于盐水组。(2)股骨远端骨密度及胫骨近端软骨下骨骨量:仙灵骨葆组均显著高于盐水组。(3)免疫组化:仙灵骨葆组MMP-13的表达显著低于盐水组,BMP-2的表达显著高于盐水组。[结论]仙灵骨葆125mg/(kg·d)灌胃能够部分阻止骨关节炎兔关节软骨的退变,其作用机制可能与下调MMP-13的表达,上调BMP-2的表达,同时增加软骨下骨骨量,改善其微观结构有关。[Objective]To assess the protective effects of Xianlinggubao on articular cartilage and subchondral bone in experimental osteoarthritis induced by anterior cruciate ligament transaction （ACLT）in rabbits. [Methods]Thirty healthy Japanese white rabbits were divided randomly into three groups：sham group,ACLT＋XLGB group and ACLT＋ NS group. Rabbits in ACLT＋XLGB group and ACLT＋NS group received anterior cruciate ligament transection（ACLT）on the right knee. Rabbits in sham group received untreatment as control. ACLT＋XLGB group received a daily administration of XLGB at a dose of 125 mg/kg of body weight for 6 weeks.In contrast,ACLT＋NS group received saline at the same dose. All rabbits were sacrificed at 6 weeks after operation. The right femora were removed,measured for bone mineral density （BMD）,and then were observed for the macro-pathologic changes with HE staining and graded by Mankin＇s scale. Expression levels of BMP-2 and MMP-13 were detected by immunostaining. The subchondral bone of right tibial plateau was cut and stained for the measurement of bone histomorphometry.[Results]（1）The Mankin＇s score in ACLT＋ XLGB group was significantly lower than that in ACLT＋ NS group （P〈0.05）. （2） The BMD of distal femora and subchondral bone mass of proximal tibia in ACLT＋ XLGB group was significantly higher than that in ACLT＋NS group （P〈0.05）. （3） In ACLT＋XLGB group：the expression level of MMP-13 was significantly lower while BMP-2 was significantly higher than that in ACLT＋NS group （P〈0.05）.[Conclusion]Daily administration of XLGB at a dose of 125 ug/kg/d for 6 weeks can partially decrease the expression level of MMP-13 and increase the expression level of BMP-2.XLBG probably protects articular cartilage by improving the structure of subchondral bone and reducing the degradation of cartilage matrix.

关 键 词：仙灵骨葆 骨性关节炎 软骨细胞 软骨下骨 BMP-2 MMP-13 

分 类 号：R285.5[医药卫生—中药学]