复方丹参注射液对兔内毒素休克的防治作用  

Protective effects of composite salvia miltiorrhiza injection on endotoxic shock in rabbits

作　　者：黄青青[1] 杨镛[1] 万林骏[1] 苏美仙[1] 文士铭[1] 

机构地区：[1]昆明医学院第二附属医院

出　　处：《中国危重病急救医学》1998年第4期227-230,共4页Chinese Critical Care Medicine

摘　　要：目的：通过测定兔内毒素休克时肿瘤坏死因子（ＴＮＦ）和自由基的变化，探讨复方丹参注射液对兔内毒素休克的防治作用。方法：健康家兔２４只随机分为３组，每组８只。对照组（Ａ组）输入与其它２组等量的０．９％氯化钠液；内毒素组（Ｂ组）静注精制内毒素；防治组（Ｃ组）于注入内毒素前输入复方丹参注射液。实验期间定时测定ＴＮＦ、丙二醛（ＭＤＡ）含量和超氧化物歧化酶（ＳＯＤ）活性，同时监测平均动脉压（ＭＡＰ）、心率（ＨＲ）和计实验７小时兔存活数。结果：在整个实验时间内，Ａ组各项指标均保持稳定（Ｐ均＞０．０５）；Ｂ组ＴＮＦ和ＭＤＡ均升高（Ｐ均＜０．０５），ＳＯＤ活性降低（Ｐ均＜０．０５），ＭＡＰ、ＨＲ均下降（Ｐ均＜０．０５）；Ｃ组ＴＮＦ和ＭＤＡ升高程度均远低于Ｂ组（Ｐ均＜０．０５），ＳＯＤ活性保持稳定（Ｐ均＞０．０５）；实验７小时Ａ、Ｃ组各存活８只兔，而Ｂ组仅存活１只兔。结论：ＴＮＦ及自由基均参与了内毒素休克病理变化过程；复方丹参注射液能明显降低ＴＮＦ的增加幅度，减轻自由基造成的脂质过氧化作用，并能对抗内毒素休克时ＳＯＤ活性降低现象。Objective:To investigate the changes in tumor necrosis factor (TNF) and free radical (FR) after endotoxin challenge and the protective effects of composite salvia miltiorrhiza injection on endotoxic shock in rabbits.Methods:Twentyfour healthy rabbits were randomly divided into 3 groups (8 animals in each group).In control group (group A),animals were infused with the same amount of 09% sodium chloride as the other 2 groups.Animals received intravenously E.coli endotoxin in endotoxic group (group B),and composite salvia miltiorrhiza injection prior to the injection of endotoxin in treatment group (group C).Plasma TNF,malondialdehyde (MDA),superoxide dismutase(SOD) were measured at various time points.Also,mean arterial pressure (MAP),heart rate (HR) and survival rate at 7 hours in each group were recorded.Results:Throughout the observation period,all parameters in group A remained stable (all P>005).In group B,plasma TNF and MDA levels markedly increased (all P<005),while the activity of SOD,HR and MAP decreased (all P<005).In group C,however,the increase of TNF and MDA was significantly less than that in group B (all P<005),and activity of SOD kept relatively stable (all P>005).In addition,MAP in group C decreased during initial phase,but tended to recover afterwards (all P<005).All animals survived in both group A and group C,only 1 animal survived in group B.Conclusions:These data suggest that TNF and FR are involved in the pathophysiological alterations of endotoxic shock.Composite salvia miltiorrhiza injection can markedly inhibit TNF and FR formation and the decrease of SOD activity following endotoxin challenge,and it may have beneficial effects on improvement of survival rate in rabbits suffering from septic shock.

关 键 词：内毒素休克 肿瘤坏死因子 自由基 丹参注射液 

分 类 号：R631.405[医药卫生—外科学]