白羊草真叶愈伤组织诱导和植株再生  

Callus Induction and Plant Regeneration from Euphyllas of Bothriochloa ischaemum

作　　者：董宽虎[1] 于娜[1] 程钰宏[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《草地学报》2009年第2期141-144,共4页Acta Agrestia Sinica

基　　金：国家科技支撑计划项目(2007BAD56B01);农业部公益性行业(农业)科研专项(nyhyzx07-022)资助

摘　　要：以7日龄白羊草（Bothriochloa ischaemum（L.）Keng）真叶作为外植体,进行愈伤组织诱导和植株再生研究,筛选较合适的激素组合,以建立白羊草再生体系。结果表明：在愈伤诱导阶段KT的作用明显,其与2,4-D的交互作用优于ABA与2,4-D,也高于单独使用2,4-D,在2,4-D1.0 mg/L＋KT0.5 mg/L时诱导率最大,达到93.45%;分化阶段6-BA0.1 mg/L与NAA 0.04~0.06 mg/L的激素组合中分化率差异不明显。The feasible hormone combinations were selected for callus induction and plantlet regeneration of Bothriochloa ischaernurn （L.） Keng. using the 7-day old euphyllas of as explants,in order to build plamtlet regeneration system. The results show that the effect of KT was in evidence on callous induction, the in- teraction of KT and 2,4-D was superior than that of ABA and 2,4-D or 2,4-D only. The maximal callus induction rate reached 93. 45% when the callus induction medium supplemented with 1.0 mg L-1 2,4-D and 0.5 mg L-1 KT. When the callus were transferred to the medium containing 0.1 mg L-1 6-BA and 0.04 0.06 mg L-1 NAA, the differentiation frequency of shoot was not significantly different.

关 键 词：白羊草 真叶 愈伤诱导 植株再生 

分 类 号：Q943.1[生物学—植物学]