巯基苯胺聚合膜结合亲和素生物素放大的阻抗免疫传感器  

Electrochemical impedance immunosensor based on aminobenzenthiol polymerization film and avidin-biotin amplification

作　　者：严八妮[1] 张云[1] 王桦[1] 沈国励[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《化学传感器》2009年第1期16-20,共5页Chemical Sensors

摘　　要：将巯基苯胺(p-aminobenzenthiol,p-ABT)组装于金电极表面,进而电聚合形成稳定、致密、有序的p-ABT纳米复合膜界面,固定功能化的碳纳米管后,再通过引入亲和素-生物素放大系统,将生物素化羊抗人IgG固定于电极表面,用以检测hIgG,建立了一种高灵敏的电化学免疫阻抗传感器,获得传感器的线性范围为0.01～100ng/mL,检测下限为0.075ng/mL(R/N=3)。p -aminobenzenthiol （ p -ABT ） was self-assembled on the surface of the gold electrode, then was electrochemical polymerizational deposited resulting in a stable, dense and ordered p-ABT nanocomposite film on the interface of the electrode. After the immobility of functionalized carbon nanotubes （CNTs）, biotinted-goat antihuman IgG was modified on the electrode surface by introducing the biotin-avidin system（BAS） to detect human IgG. An electrochemical impedance immunosensor with favorably high sensitivity was founded. The linear range of the resulted immunosensor was observed from 0.01 to 100 ng/mL with a detection limit of 0.075 ng/mL （R/N=3）.

关 键 词：对巯基苯胺 电聚合 亲和素生物素 阻抗免疫传感器 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]