功能化硅壳磁性纳米颗粒亲和吸附介质靶向分离蛋白质研究  

Research on target protein separation with affinity adsorbents based on functionalized silica coated magnetic nanoparticles

作　　者：霍希琴[1] 陈英杰[1] 何晓晓[1] 谭蔚泓[1] 

机构地区：[1]化学生物传感与计量学国家重点实验室,化学化工学院,生物医学工程中心,湖南大学,生物纳米与分子工程湖南省重点实验室,湖南长沙410082

出　　处：《化学传感器》2009年第1期36-41,共6页Chemical Sensors

基　　金：教育部科学技术重点项目(批准号:107084);新世纪优秀人才支持计划(批准号:NCET-06-0697)

摘　　要：基于溴化氰修饰方法在硅壳磁性纳米颗粒表面修饰功能性生物分子,获得功能化硅壳磁性纳米颗粒亲和吸附介质。以胰蛋白酶-胰蛋白酶抑制剂作为模式亲和对,基于酶和抑制剂之间的特异性结合原理,首先考察了胰蛋白酶抑制剂的修饰对硅壳磁性纳米颗粒粒径和电位的影响及其修饰效率,然后利用胰蛋白酶抑制剂修饰的硅壳磁性纳米颗粒亲和吸附介质对简单模型蛋白质混合溶液以及胰脏组织中的胰蛋白酶进行靶向分离。该研究基于硅壳磁性纳米颗粒的内核磁性及表面生物修饰,为蛋白质分离提供了一种新型亲和吸附介质。Functionalized silica coated magnetic nanoparticles were developed as affinity protein adsorbents, which were activated with CNBr and modified by biomoleculars. Duo to the differential recognition between trypsin and trypsin inhibitor, they were chosen as representation for the affinity couple. And trypsin inhibitor was modified on the silica coated magnetic nanoparticles. The effects of modification on the size and zeta potential of silica coated magnetic nanoparticles and the modification efficiency were studied. And trypsin in simple model protein mixture or pig pancreas was objectively separated with the biofunctionalized silica coated magnetic nanoparticles. This study provided a new kind of affinity adsorbents for protein separation with silica coated magnetic nanoparticles, which has magnetic core and biomodified surface.

关 键 词：硅壳磁性纳米颗粒 亲和吸附 蛋白质 分离 

分 类 号：Q51-3[生物学—生物化学]