鸟嘌呤和8-羟基脱氧鸟嘌呤核苷在聚甘氨酸修饰电极上的电化学行为及其同时测定  

Electrochemical behavior and simultaneous determination of guanine and 8-hydroxy-2'-deoxyguanosine at a polyglycine modified electrode

作　　者：李艳芬[1] 张晶晶[1] 赵倩[1] 梅艳丽[1] 潘壮英[1] 贾文丽[1] 陶绪泉[2] 王怀生[1,2] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059 [2]聊城大学材料科学与工程学院,山东聊城252059

出　　处：《化学传感器》2009年第1期42-48,共7页Chemical Sensors

基　　金：国家自然科学基金(20475024;20775031)

摘　　要：在优化的实验条件下,利用电化学方法制备了甘氨酸修饰电极,对修饰膜的电活性进行了表征。用循环伏安法研究了鸟嘌呤(G)和8-羟基脱氧鸟嘌呤核苷(8-OH-dG)在聚甘氨酸修饰电极上的电化学行为,并建立了对两者进行分别检测和同时检测的分析方法。实验结果表明,聚甘氨酸修饰电极可以增强鸟嘌呤和8-羟基脱氧鸟嘌呤核苷在电极表面的吸附,并且可以加快鸟嘌呤和8-羟基脱氧鸟嘌呤核苷在电极表面的电子传输,使两种电活性物质在聚甘氨酸修饰电极上的电化学信号明显增大,检测灵敏度大大提高,并且该修饰电极具有良好的稳定性和重现性,可用于鸟嘌呤和8-羟基脱氧鸟嘌呤核苷的分别和同时检测。In this paper, a polyglycine modified electrode was prepared by electrochemical polymerize glycine under the optimized experimental conditions. The electric activity of modified membranes was characterized. And the electrochemical behavior of guanine （G） and 8-hydroxy-deoxy guanosine （8-OH-dG） were studied with CV technique at the modified electrode. We set up an analytical detection method to determine guanine and 8-OH-dG separately and simultaneously. Experimental results show that the polyglycine modified electrode can enhance the adsorption of guanine and 8-OH-dG and speed up the electronic transmission at the electrode surface which contribute the electrochemical signal increases markedly, and the detection sensitivity improved either. The modified electrode gives good stability and reproducibility, which good factors give a satisfied result in determination of guanine and 8-OH-dG respectively and simultaneously.

关 键 词：鸟嘌呤 8-羟基脱氧鸟嘌呤核苷 聚甘氨酸修饰电极 玻碳电极 同时测定 

分 类 号：O657.1[理学—分析化学]