葛根素对大鼠酒精性肝炎的影响及其机制  

Protective effect of puerarin on rats with alcoholic hepatitis

作　　者：曲智威[1] 温春阳[1] 王爱平[1] 鞠文博[2] 任爱华[2] 刘卯阳[2] 周艳[2] 

机构地区：[1]北华大学附属医院消化内科,吉林省吉林市132011 [2]北华大学解剖教研室,吉林省吉林市132011

出　　处：《世界华人消化杂志》2009年第7期657-661,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨葛根素对酒精性肝炎的作用机制.方法:21只Wistar大鼠,随机分为3组,正常对照组(n=7)用玉米油+500g/L葡萄糖20mL/(kg?d)灌胃,模型组(n=7)用400mL/L乙醇按8g/(kg?d)+玉米油灌胃,葛根素组(n=6):葛根素+酒精,按葛根素5mg/(kg?d)腹腔注射给药.采用比色法测定血清AST,ALT及GST,放免法测定血浆PGE2,TNF及IL-6,Western blot方法检测肝组织COX-2表达,并通过光镜观察肝组织病理变化.结果:血浆ALT,AST及GST模型组与正常对照组比较有明显升高(107.5±6.81vs33.20±10.55,138.29±9.72vs47.86±14.3,3.57±0.53vs1.43±0.43,均P<0.01);与模型组比较,葛根素组血浆ALT及AST水平(52.33±13.19,63.33±7.03)有明显下降(P<0.01).GST有下降趋势,但无明显著意义;模型组与正常对照组比较血浆PGE2,TNF及IL-6明显上升(274.13±26.15vs193.84±23.97,1.85±0.11vs0.90±0.18,68.07±12.64vs40.50±5.09,均P<0.01);与模型组比较,葛根素组血浆PGE2,TNF及IL-6明显下降(227.05±21.55,1.35±0.19,53.16±5.62,均P<0.01),COX-2正常对照组呈弱表达,模型组显著高表达,与正常对照组比较差别显著(P<0.01),葛根素组COX-2表达下调,与模型组相比有明显下调(F=27.94,P<0.01).结论:葛根素通过抑制肝脏COX-2的表达,从而减少炎症介质PGE2、TNF及IL-6的生成,最终减轻酒精对肝脏的损伤作用.AIM： To investigate mechanism of puerarin on alcoholic hepatitis in rats.METHODS： Twenty-one Wistar rats were divid- ed into three groups randomly, with intragastric administration of the control group by corn oil ＋ 500 g/L glucose 20 mL/（kg·d）; intragastric administration of the model group by 400 mL/L alcohol 8 g/（kg ~ d）＋ corn oil; peritoneal injection of the puerarin group by puerarin 5 rag/（kg · d）; After thirty days. Serum and liver tissue samples were prepared, and the levels of alanine transaminase （ALT）, aspartate transaminase （AST）, and y-glutamyltranspeptidase （GST） were determined by chromatometry, serum PGE2, TNF-α and IL-6 by radioimmunity method, and COX-2 expression on the hepatic tissue by Western blot.Pathological changes on hepatic tissue was determined by light microscopy.RESULTS： The serum ALT, AST and GST was significantly higher in model group than in con- trol group （107.5 ± 6.81 vs 33.20 ± 10.55, 138.29 ± 9.72 vs 47.86 ± 14.3, 3.57 ± 0.53 vs 1.43 ± 0.43, all P 〈 0.01）. The serum ALT and AST was signifi- cantly lower in puerarin group than in model group （52.33 ± 13.19, 63.33 ± 7.03, P 〈 0.01）. The serum GST level was lower, but not signifi- cantly. The levels of serum PGE2, TNF-α and IL-6 were significantly higher in model group than in control group （274.13 ± 26.15 vs 193.84 ± 23.97, 1.85 ± 0.11 vs 0.90±0.18, 68.07 ± 12.64 vs 40.50 ± 5.09, all P 〈 0.01）, and the levels of the serum PGE2, TNF-α and IL-6 were significantly lower in puerarin group than in model group （227.05 ± 21.55, 1.35 ± 0.19, 53.16 ± 5.62, all P 〈 0.01）. Expression of COX-2, was weak, but had high expression in model group（P 〈 0.01）. The expression of COX-2 in puerarin group was down regulated, compared with model group （P 〈 0.01）.CONCLUSION： Puerarin has effects on hepatic injury in alcoholic hepatitis.

关 键 词：葛根素 酒精性肝炎 前列腺素2 肿瘤坏死 因子 白介素6 环氧化物酶2 

分 类 号：R285.5[医药卫生—中药学]