基于AFLP技术对中国虾夷扇贝群体种质资源的研究  

Studies of Japanese Scallop (Mizuhopecten yessoensis) Germplasm by Using AFLP Markers

作　　者：鲍相渤[1] 董颖[1] 赫崇波[1] 高祥刚[1] 刘卫东[1] 

机构地区：[1]辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室

出　　处：《生物技术通报》2009年第4期126-129,共4页Biotechnology Bulletin

基　　金：国家留学人员择优资助项目(LXZZ2005001)

摘　　要：利用AFLP技术对国内较具代表性的5个群体与日本群体虾夷扇贝相比照进行遗传多样性的分析。采用6对引物组合对6个群体178个个体进行扩增,共得到308个位点,6个群体的多态位点比例为62.66%～69.81%,其中日本群体最高,小长山群体最低,且呈显著性差异;香农氏指数为0.337～0.374,其中日本群体最高,小长山群体最低。凌水桥群体与旅顺群体间遗传相似性最高(0.9810),小长山群体与凌水桥群体间遗传相似性最低(0.9641);相对的遗传距离的计算结果与遗传相似性结果一致。数据分析表明养殖方式对虾夷扇贝遗传多样性影响不大。本试验结果为我国虾夷扇贝种质遗传多样性维持、保护和可持续利用提供参考。Genetic diversity of five Japanese scallop（Mizuhopecten yessoensis） populations in China and one population from Japan was conducted by using amplified fragment length polymorphism（AFLP）. Total 308 reproducible bands amplified with 6 AFLP primer combinations were obtained from 178 individuals. Among the six populations, the one with the greatest percentage of polimorphic loci （69.81% ） and the highest Shannon＇ s Information index（0. 374） was the Japan population and the lowest was the Xiaochang Mountain population（62.66%, 0. 337 ）. The genetic similarity between Lingshui Bridge population and Lvshun population was the largest （0. 9810） , and the Xiaochang Mountain population and Lingshui Bridge population was the lowest（0. 9641 ）. The values of the genetic distances pointed to the same conclusion. The result of this study showed that culturing operations had not brought a significant effect on the genetic diversity of Japanese scallop.

关 键 词：虾夷扇贝 AFLP 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]