HLA—A Bw4表位与HIV-1病毒载量和CD4^＋T淋巴细胞计数关系的研究  

The association between HLA-A antigen carrying Bw4 epitope and HIV-1 viral loads and CD4^＋ T cell counts

作　　者：陈健平[1] 贾明明[1] 刘宏伟[1] 刘沙[1] 张小青[1] 赵红静[1] 邵一鸣 洪坤学[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京100050

出　　处：《中华检验医学杂志》2009年第4期383-387,共5页Chinese Journal of Laboratory Medicine

基　　金：国家重点基础研究发展计划（2006CB504207）

摘　　要：目的研究含Bw4表位的HLA—A抗原在HIV-1感染者疾病进展中的作用。方法应用高分辨率人类白细胞抗原（HLA）分型方法对340例HIV-1感染者进行HLA-A和HLA—B位点的等位基因分型，确定Bw4表位的携带情况，分析HLA—A和HLA—B携带Bw4表位与感染者CD4^＋T淋巴细胞计数和血浆病毒载量的关系。结果与不携带Bw4者（0Bw4）（CD4^＋T淋巴细胞计数：中位数294个／μl，病毒载量：中位数6．29×10^4拷贝／ml）相比，IBw4-A基因型者和2Bw4-AA基因型者的CD4^＋T淋巴细胞计数水平（分别为307和308个／μl）基本相当，而病毒载量（分别为1．53×10^5和2．68×10^5拷贝／m1）有增高趋势。与不携带Bw4表位的感染者相比，仅在HLA—B抗原中含Bw4表位的HIV-1感染者的CD4^＋T淋巴细胞计数（417个／μl）显著增高（P=0．013），而病毒载量（2．10×10^4拷贝／ml）显著降低（P=0．007）。结论含有Bw4表位的HLA-B抗原在HIV-1感染中具有延缓疾病进展的作用，而含有Bw4表位的HLA-A抗原对HIV-1感染者未见保护性作用。Objective To investigate the impact of HLA-A antigens carrying Bw4 epitopes on disease course of HIV-1 infection. Methods Three hundred and forty subjects chronically infected with HIV-1 were recruited and their HLA-A and B alleles were genotyped with sequence-based high resolution typing assay. HLA-Bw genotypes of these HIV-1 infected subjects were determined and their association with CD4^＋ T cell counts and viral load were analysed. Results When compared with subjects carrying no Bw4 epitopes in HLA-A and HLA-B loci （0Bw4） （median of CD4^＋ T cell counts：294/μl; plasma viral load median 6. 29 ×10^4 copies/ml）, CD4^＋ T cell counts in subjects with genotypes of 1Bw4-A and 2Bw4-AA were comparable （307 and 308/μl, respectively） , but higher viral load （ 1.53×10^5 and 2. 68 ×10^5 copies/ml, respectively） was observed. In subjects with Bw4 epitopes in HLA-B alleles but no in HLA-A, significantly higher CD4^＋ T cell counts （417/μl, P = 0. 013 ） and lower viral load （2. 10 ×10^4 copies/ml, P = 0. 007 ） were observed compared with those without Bw4 in HLA-A and HLA-B. Conclusion HLA-B antigens carrying Bw4 epitope were protective in HIV-1 infection by maintaining higher CD4^＋ T cell counts and lower viral load, hut such protective effect was not observed in HLA-A antigens carting Bw4 epitope.

关 键 词：获得性免疫缺陷综合征 HLA—A抗原 HIJA-B抗原 HIV-1 病毒载量 CIM淋巴细胞计数 

分 类 号：R686[医药卫生—骨科学]