医用生物蛋白胶携载更昔洛韦体外缓释研究  

Experimental study on in-vitro sustain-release of medical biofibrin glue carrying Ganciclovir

作　　者：余涛[1] 钟玲[1] 曲斐[1] 偦陶 尚京川[1,3] 

机构地区：[1]重庆医科大学药学院药物分析教研室,重庆400016 [2]重庆市第五人民医院药剂科,重庆430062 [3]重庆医科大学医药研究所,重庆400016

出　　处：《重庆医科大学学报》2009年第3期326-328,共3页Journal of Chongqing Medical University

摘　　要：目的:建立HPLC测定体外类体环境中更昔洛韦浓度的方法,并考察医用生物蛋白胶携载更昔洛韦在体外的缓释作用。方法:用医用生物蛋白胶携栽更昔洛韦,观察缓释情况并测定其释放率。HPLC流动相为水∶甲醇(2∶98);流速:1ml/min;检测波长:252nm;柱温:30℃。结果:更昔洛韦在5～350μg/ml范围内线性关系良好;日内和日间精密度RSD为0.89%～2.35%,高、中、低3个浓度的回收率为98.4%～100.8%.该方法灵敏度高,重复性好,可适用于在体外条件下更昔洛韦浓度的测定。结论:医用生物蛋白胶携栽更昔洛韦在体外类体环境下缓释效果良好。Objective：To establish an HPLC method for the determination of the concentration of ganciclovir in vitro, and to inspect sustain-release of medical biofibrin glue carrying Ganciclovir. Methods：The HPLC system consisted of water-methanol （98：2） as mobile phase with the flow rate of 1.0 ml/min,and the detection wavelength was 252 nm and the injection volumn was 20 μl. Column tempetature was 30℃. Results：The linear range of ganciclovir was 5-350 μg/ml （r=0.9996）, displaying a good linear relationship. The within-day and between-days relative standard deviations（ RSD ） were 0.89% - 2.35%. The methodological recovery ranged from 98.4 % to 100.8 %. The elaborated method was sensitive, precise and applicable for determining the concentration and release rate of ganciclovir in vitro. Conclusion： The sustain-release eflhct of medic, al biofibrin glue carrying ganciclovir in vitro is good.

关 键 词：医用生物蛋白胶 更昔洛韦 体外缓释 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]