维拉帕米对梗阻性黄疸大鼠肝组织核因子κB表达的影响  

Effect of verapamil on expression of NF-κB in liver of obstructive jaundice rats

作　　者：禹良国[1,2] 舒新军[1,2] 陶政[1,2] 李杰[2] 徐三荣[2] 

机构地区：[1]江苏大学临床医学院,江苏镇江212001 [2]江苏大学附属医院普外科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2009年第2期135-138,I0002,共5页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:探讨维拉帕米对梗阻性黄疸时肝组织核因子-κB(nuclear factor kappa B,NF-κB)表达的影响。方法:将雄性SD大鼠48只随机分成胆管结扎组、维拉帕米组和假手术组。分别于术后第7天、14天处死大鼠,观察肝组织NF-κB p65表达、丙二醛(MDA)和超氧化物歧化酶(SOD)含量以及血清肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的变化。结果:维拉帕米组肝组织NF-κB p65表达、MDA含量及血清TNF-α,IL-6水平均明显低于胆管结扎组(P<0.05或P<0.01)。结论:维拉帕米可下调肝组织NF-κB的表达,减轻梗阻性黄疸时肝损伤。Objective： To investigate the effect of verapamil on expression of nuclear factor kappa B （NF-κB） in the liver during obstructive jaundice. Methods： Forty eight male Sprague-Dawley rats were randomly divided into three groups： bile duct-ligation group, bile duet-ligation plus verapamil group and sham-operation group. The rats were sacrificed at 7 and 14 days after operation. The following parameters were measured： expressions of hepatic NF-κB p65, contents of maleicdialdehyde （MDA） and superoxide dismutase（SOD） in hepatic tissue, tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） in serum. Results ： Expressions of NF-κB p65, contents of MDA and levels of serum TNF-α, IL-6 in verapamil group were all obviously lower than those in bile duct-ligation group（P 〈 0.05 or P 〈0.01 ）. Conclusion： Verapamil may protect against the liver injury in rats with obstructive jaundice through reducing the expression of NF-κB.

关 键 词：维拉帕米 梗阻性黄疸 核因子-ΚB 肝损伤 

分 类 号：R333.4[医药卫生—人体生理学] R575[医药卫生—基础医学]