稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布  

Distribution of Two Blast Resistance Genes Pita and Pib in Major Rice Cultivars in China

作　　者：时克[1] 雷财林[1] 程治军[1] 许兴涛[1] 王久林[1] 万建民[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《植物遗传资源学报》2009年第1期21-26,共6页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划(2006BAD13B01-3);"948"项目(2006-G61)

摘　　要：主效抗稻瘟病基因Pita和Pib在我国很多稻区表现高水平的稻瘟病抗性,被广泛应用于我国的水稻育种和生产。但这2个基因在国内主栽品种中的分布及利用情况一直缺乏详细的资料,致使育种利用上存在着盲目性。本研究利用源于Pita和Pib基因本身的特异性分子标记,结合稻瘟病菌接种鉴定,检测和分析了我国58份水稻主栽品种(杂交稻亲本)的Pita和Pib抗性基因型。结果表明,特籼占25、佳禾早占、密阳46、测64-7等4个籼稻品种携带Pita和Pib2个基因;籼小占等4个籼稻品种(系)和早丰9号等5个粳稻品种携带抗性基因Pita;绵恢501等5个籼稻品种(系)和粳稻品种武育粳7号、辽粳454携带抗性基因Pib。The two major blast resistance genes, Pita and Pib, showed good resistance spectrum to Magna- porthe grisea isolates in many rice-growing areas of China, and has been utilized in rice breeding and production. However, the distribution of this two genes in present rice leading cultivars has not been well investigated, resulting in the blindness of their utilization in rice breeding. In the present study, the existence of the two genes in 58 rice leading cultivars or hybrid rice parents were rapidly determined using the dominant DNA marker of Pita/pita and Pib/pib alleles. Both Pita and Pib genes was found in four indica cultivars, i. e. , Texianzhan 25, Jiahezaozhan, Miyang 46 and Ce64-7. The Pita alone was found in other four indica and five japonica cultivars/lines, and the Pib alone in other five indica and two japonica cultivars/lines. These results are useful for rational utilization and deployment of the rice leading cultivars/lines with the Pita and Pib genes, and for incorporating and stacking these genes into elite present leading cuhivars/lines by marker-assisted selection in rice breeding programmes nationwide.

关 键 词：水稻 稻瘟病 主栽品种 抗性基因 分子标记 

分 类 号：S511[农业科学—作物学]