刺槐不同居群遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Robinia pseudoacacia Populations

作　　者：孙芳[1] 杨敏生[1] 张军[1] 谷俊涛[1] 

机构地区：[1]河北农业大学林学院,保定071001

出　　处：《植物遗传资源学报》2009年第1期91-96,共6页Journal of Plant Genetic Resources

基　　金：国家林业局"948"项目(2005-4-41)资助

摘　　要：利用ISSR标记对全国10个刺槐居群子代100个个体的遗传多样性进行了比较分析,从65个随机引物中筛选出10个多态性引物进行扩增,共检测到91个位点,多态位点数(AP)为85,多态位点百分率(P)为93.41%。刺槐在种级水平的遗传多样性参数略高于居群水平,多态位点百分率(P)分别为95.60%、69.01%,Shannon′s信息指数(I)分别为0.6145、0.3733,Nei′s基因多样性指数(H)分别为0.4337、0.2514。居群间的遗传分化指数Gst、Nei′s基因多样性指数和Shannon′s信息指数统计结果,均显示出中国刺槐居群内遗传多样性大于居群间遗传多样性。利用PopGen32软件对10个居群进行聚类分析可知,10个刺槐群体可分为三大类,亲缘关系和地理分布呈一定的相关性,但没有形成明显的地理变异模式。ISSR molecular markers were used to detect genetic diversity of 100 individuals in 10 populations of Robinia pseudoacacia. Ten ISSR primers were selected and 91 polymorphic bands were induced. Percentage of polymorphic bands is 93.41%. As for Robinia pseudoacacia at species level, the mean Nei＇s gene diversity index （H） was 0. 4337, the Shannon＇s information index （1） was 0. 6145, indicating that in the 10 Robinia pseudoacacia populations, the genetic differentiation among populations was relatively small, whereas within populations was relatively greater. The dendrogram of genetic relation was constructed on the basis of genetic distance among 10 populations. Groups cluster analysis based on the UPGMA method indicated that although the 10 populations could be divided into 3 groups and the Robinia pseudoacacia seed sources was less correlated with geographic latitude. There＇s no evident geographic patterns of the Robinia pseudoacacia seed sources happened.

关 键 词：刺槐 居群 遗传多样性 遗传结构 ISSR 

分 类 号：S792.27[农业科学—林木遗传育种]