中医“热毒血瘀证”模型大鼠血管内皮损伤及血小板功能障碍研究  

Study of Vascular Endothelial Cells Injury and Platelet Dysfunction in Rats with Noxious Heat and Blood Stasis Syndrome

作　　者：江欢[1] 周岩[2] 孙晓红[2] 徐群渊[2] 

机构地区：[1]首都医科大学 [2]首都医科大学北京神经科学研究所

出　　处：《中西医结合心脑血管病杂志》2009年第4期425-428,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家重点基础研究发展计划(973计划)基金项目(No.2003CB517104)

摘　　要：目的研究具有中医"热毒血瘀证"表征的大鼠血管内皮细胞(VEC)损伤和血小板功能的病理变化,以阐释中医"热毒血瘀证"的现代生物医学基础。方法大鼠腹腔注射内毒素即脂多糖(LPS),连续8周,在不同时间点进行行为学分析、检测舌血氧饱和度基础上,检测血浆内皮素(ET)、热休克蛋白-70(HSP70)、血栓烷B2(TXB2)和6-酮-前列腺素(6-Keto-PGF1α)的变化。结果注射毒素后大鼠活动减少,舌血氧饱和度降低。早期血浆ET、HSP70和TXB2/6-Keto-PGF1α增高,晚期TXB2/6-Keto-PGF1α降低。结论具有舌体组织血氧饱和度下降的中医"热毒血瘀证"存在血管内皮细胞损伤及血小板功能障碍,VEC损伤在中医"热毒血瘀证"发展过程可能起着重要作用。Objective To investigate the injury of vascular endothelial cells and dysfunction of blood platelet in rats with traditional Chinese medicine syndrome of noxious heat and blood stasis. Methods The lipopolysaccharide （LPS） was injected peritoneally and continuously to SD rats for 8 weeks. The behavior was analysis and blood oxygen saturation of the tongue was studied during the injections. The plasma endothelin （ET）, heat shock protein 70 （HSP70）, thromboxane - B2 （TXB2） and prostaglaudin - 1 a （6 - Keto - PGF1α ） were detected. Results After LPS injections,the behavioral activities of rats were reduced and the oxygen saturation in the tongue was lowered. The ET,HSP70, and TXB2/6 - Keto -PGF1α in the plasma were increased in the early stage of the injection and the TXB2/6 - Keto - PGF1α, was deereased late. Conclusion The injury of the vascular endothelial cells and blood platelet function were proved to be the important factors for elucidating the principle of traditional Chinese medicine syndrome of noxious heat and blood sta sis,which was further confirmed by tongue tissue blood oxygen saturation in present study.

关 键 词：热毒血瘀证 内毒素 脂多糖 血栓烷B2 

分 类 号：R255.7[医药卫生—中医内科学] R285.5[医药卫生—中医学]