实验性脑出血大鼠脑内ICAM-1表达及脑溢安的保护作用  

The Effects of Naoyi'an on the Expression of ICAM-1 in the Brain of Rats with Experimental Intracerebral Hemorrhage

作　　者：智屹惠 黎杏群[2] 李彤[3] 万赛英[2] 唐涛[2] 

机构地区：[1]浙江中医大学附属第一医院,杭州310006 [2]中南大学湘雅医院中西医结合研究所 [3]浙江大学医学院第一附属医院

出　　处：《浙江中医药大学学报》2009年第2期158-160,共3页Journal of Zhejiang Chinese Medical University

基　　金：国家中医药管理局资助课题(No:2000-J-B-03)~~

摘　　要：[目的]研究实验性脑出血大鼠脑内ICAM-1表达的变化及脑溢安对其的影响。[方法]Ⅶ型胶原酶立体定位法建立大鼠脑出血模型,分别观察ICAM-1原位杂交和Western blot的变化及脑溢安对其的干预作用。[结果]模型组可见ICAM-1表达增强,脑溢安能明显降低ICAM-1的表达(P<0.01)。[结论]实验性脑出血大鼠脑内ICAM-1表达增强,脑溢安对其具有保护作用。[Objective] To investigate the effects of Naoyi＇an （NYA） on the expression of ICAM-1 in the brain of rats with experimental in tracerebral hemorrhage. [Methods] The rats model of intraeerebral hemorrhage was prepared by type Ⅶ collagenase injected into globus pallidus under stereotaxic apparatus. Then,the expression of ICAM-1 with or without intervention with NYA was determined in the brain of these animals respectively by in situ hybridization and western blot technique. [Results] Among the modeling animals, the expression of ICAM-1 was increased after the operation. In contrast, the expression of ICAM-1 was significantly down-regulated in the rats treated by NYA（P〈0.01）. [Conclusion] The ICAM-1 expression was significantly up-regulated in the brain of rats with intracerebral hemorrhage, while NYA can down regulate the expression of ICAM-1.

关 键 词：脑出血 ICAM-1 脑溢安 大鼠 

分 类 号：R285.5[医药卫生—中药学]