生长调控因子与GHRP-6微球的制备及对水貂生长的影响  

Effects on mink growth of poly(D,L-lactide-co-glycolide) microspheres containing growth regulating factor and gHRP-6

作　　者：张永亮[1,2] 章倩倩[3] 侯锋[4] 张明军[1] 戴建威[5] 刘松财[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]华南农业大学动物科学学院,广东广州610640 [3]中山大学生物工程研究中心教育部基因工程重点实验室,广东广州510275 [4]军事医学科学院军事兽医研究所,吉林长春130062 [5]广州医学院生物化学教研室,广东、广州510182

出　　处：《中国兽医学报》2009年第4期456-459,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270973)

摘　　要：以乳酸-乙醇酸共聚物为载体,利用复乳-液中蒸发法制备pcDNA3-GRF微球、pIRES-HBs/AgSSM微球和GHRP-6微球。将空微球、pcDNA3-GRF微球(1 mg/kg),pIRES-HBs/AgSSM微球(1 mg/kg)和GHRP-6微球(1mg/kg)分别肌肉注射至水貂骨骼肌。120 d后,3个试验组水貂体长均高于空微球组(P<0.01);采用RIA法测定各组水貂血清IGF-Ⅰ浓度,3个试验组水貂血清IGF-Ⅰ浓度均比空微球组高,但pcDNA3-GRF微球组血清IGF-I浓度显著高于对照组(P<0.05)。结果表明:pcDNA3-GRF乳酸乙醇酸共聚物微球有明显的促水貂生长作用。Injectable sustained-release pcDNA3-GRF,plRES-HBs/AgSSM and GHRP-6 microspheres were pre-pared by double emulsion-in liquid evaporation process, using biodegrable ploy lactic-co-glycolic acid as carrier. Then they were injected to skeletal muscle of minks to see the effects on mink growth. The results showed that injection of pcDNA3-GRF,pIRES-HBs/AgSSM and GHRP-6 microspheres lead to stem length were significantly higher than that of empty microspheres （P〈0.01） at 120 days postinjection. Injection of pcDNA3 GRF, pIRES-HBs/AgSSM and GHRP-6 microspheres lead to serum IGF- I levels were higher than that of empty microspheres. But the serum IGF-I levels of pcDNA3-GRF microspheres grope were significantly higher than that of the control group （P〈 0. 05）. The results above indicated that the pcDNA3-GRF microspheres could promote the growth of mink significantly.

关 键 词：乳酸-乙醇酸共聚物 微球 生长激素释放因子 生长激素释放抑制因子 生长激素释放肽-6 

分 类 号：S852.2[农业科学—基础兽医学]