两种状态抗CD_3单抗诱导人T淋巴细胞活化增殖的初步观察  

Observation of the Effects of Two Different Type CD 3McAb on Activating Human T Lymphocytes

作　　者：王立新[1] 许靖霞[2] 谭剑萍[2] 蔡仙德[2] 

机构地区：[1]南京铁道医学院医学检验系 [2]南京铁道医学院微生物学教研室

出　　处：《南京铁道医学院学报》1998年第1期12-15,共4页Journal of Nanjing Railway Medical College

基　　金：铁道部科技基金

摘　　要：目的：观察并比较粘附状态抗ＣＤ３单抗和游离状态抗ＣＤ３单抗诱导人外周血单个核细胞（ＨＰＢＭＣ）活化增殖的能力。方法：采用３Ｈ－ＴｄＲ掺入淋巴细胞增殖试验、苔盼蓝拒染法、间接免疫荧光染色法以及ＣＴＬＬ细胞ＩＬ－２检测法，分别检测人外周血单个核细胞，经两种状态抗ＣＤ３单抗刺激后，其增殖活性、外源性ＩＬ－２的协同作用、内源性ＩＬ－２释放以及ＩＬ－２Ｒ表达等生物免疫学指标。结果：粘附状态抗ＣＤ３单抗刺激ＨＰＢＭＣ，其增殖活性、活细胞总数以及增殖活性维持的时间均高于游离状态抗ＣＤ３单抗，且前者不依赖外源性ＩＬ－２；粘附状态抗ＣＤ３单抗诱导ＨＰＢＭＣ释放内源性ＩＬ－２以及细胞表面ＩＬ－２Ｒ表达的能力也强于游离状态抗ＣＤ３单抗。结论：粘附状态抗ＣＤ３单抗比游离状态抗ＣＤ３单抗具有更强的诱导人Ｔ淋巴细胞活化增殖能力。Objective:To observe and compare the effects of immobile CD 3McAb and soluble CD 3McAb on the activating and proliferating ability of resting human peripheral blood T lymphocytes in vitro .Method:The activation effects of CD 3McAb were obtained by using 3H-TdR incorporation method,trypan blue test,indirect immunofluorescence method and MTT method.Results:Immobile CD 3McAb was better than soluble CD 3McAb on mitogenesis,vital total cells and potent activity.Moreover,it was active in the absence of exogenous rIL-2.Immobile CD 3McAb can also induce HPBMC to release more endogenous IL-2 and better expressive ability of IL-2R than soluble CD 3McAb.Conclusion:Immobile CD 3McAb was more potent on activating T lymphocytes than soluble CD 3McAb.

关 键 词：抗CD3单抗 HPBMC 活化增殖 T淋巴细胞 

分 类 号：R392.11[医药卫生—免疫学]