J-Grof法直接胆红素测定中的间接胆红素反应动力学研究  

The Kinetic Studies of Indirect Bilirubin in Direct Reaction by the Jendrassik Grf Method

作　　者：张建新[1] 朱澜[2] 方宝健 

机构地区：[1]南京铁道医学院卫生分析化学教研室 [2]南京铁道医学院附属医院检验科

出　　处：《南京铁道医学院学报》1998年第1期16-18,共3页Journal of Nanjing Railway Medical College

基　　金：铁道部科技基金

摘　　要：目的：研究间接胆红素在Ｊ－Ｇｒｏｆ法直接胆红素测定中的反应动力学问题。方法：配制胆红素标准液，考察Ｂｕ（未结合胆红素或称间接胆红素）在直接反应中其反应率与稀释液酸度、蛋白质基质、预保温时间、偶氮反应时间、反应温度及Ｂｕ浓度之间的关系。结果：当稀释液酸度ｐＨ在２．０～５．０范围时，间接胆红素在Ｊ－Ｇｒｏｆ法直接反应中反应成直接胆红素的值达一平台。标本在稀释液中预保时间越短，Ｂｕ的直接反应的反应率越高，反应温度越高，偶氮反应时间越长，Ｂｕ的直接反应的反应率越高。在无稀释液时，在一定温度和偶氮反应时间下，Ｂｕ在Ｊ－Ｇｒｏｆ法直接反应中的反应率（ｘ）与其浓度Ｃ的关系基本符合ｘ＝ＡＣ－ｋ。结论：ｘ＝ＡＣ－ｋ很好地说明间接胆红素在Ｊ－Ｇｒｏｆ法直接反应中的反应率与其浓度的关系。Objective:The kinetic studies of indirect bilirubin in direct reaction by J-Grof method.Method:We studied the effects of the acidity of the diluents,protein matrix,reaction temperature,preincubation time,diazo time and the concentration of Bu on the reactivity of Bu in the direct reaction.Results:The DBIL values of the Bu standard in the direct reaction reaches a plateau in the diluents range from pH2.0 to pH5.0. The reactivity of Bu in direct reaction is higher in short preincubation time than in long preincubation time.The higher the reaction temperature is,the longer the reaction time is,the higher the reactivity of Bu in dircet reaction is.At the same temperature and same reaction time,the relationship of the reactivity( x ) of Bu and the concentration of Bu in the direct reaction without any diluents is as follows: x =A C -k (ie log x =logA-Klog C , r >-0.9928),The constant A is associated with the temperature and the diazo reaction time,and the constant K is associated with the diazo reaction time.Conclusion:The presented empirical equation, x =A C -k predicts the reactivities of Bu in direct reaction ,which were in good agreement with the obtained results.

关 键 词：间接胆红素 直接反应 动力学研究 

分 类 号：R446.112[医药卫生—诊断学]