滤膜荧光法检测大肠菌群及其与标准滤膜法的比较  

Determination of E.Coli Population with Filter Membrane Fluorometry and the Results Were Compared with Standard Filter Membrane Method

作　　者：张晖[1] 林大榕[1] 

机构地区：[1]南京铁道医学院预防医学系环境卫生学教研室

出　　处：《南京铁道医学院学报》1998年第1期23-25,共3页Journal of Nanjing Railway Medical College

摘　　要：目的：用滤膜荧光法检测大肠菌群，并将该法与标准滤膜法进行比较。方法：将菌种于肉汤培养基中培养至对数生长期，稀释，用微孔滤膜过滤，将滤膜置于含ＭＵＧＡＬ的培养液的衬垫上，置３７℃培养，在不同时间取出观察，并作荧光斑点扫描。结果：经实验室模拟水样和列车餐车水样检验，滤膜荧光法和标准滤膜法结果无差别。结论：滤膜荧光法与标准滤膜法相比具有快速。Objective:Filter membrane fluorometry(FMF) was applied the determination of total coliforms,and its results were compared with standard filter membrane method(SM).Method:Cells was cultured in broth until their logarithmic growth time come,they get soluted and filtered with micropore membrane.Then,filter membrane was put in liner containing MUGAL at 37℃,taken out/to be observed at different time,and scaned with fluorometer.Results:The results determinated with FMF were all the same as SM in the simulated water samples in the laboratory and samples in a dining car on the train.Conclusion:MF,as compared with SM,is more rapid,and MF is especially adapted to determination on the scene.

关 键 词：总大肠菌群 滤膜荧光法 标准滤膜法 β-GAD 

分 类 号：R446.5[医药卫生—诊断学]