血液稀释联合潘通预防肢体缺血再灌注损伤  

Hemodilution and pentomer prevent ischemic and reperfusion injury of limbs

作　　者：皮勇[1] 陈德杰[1] 李锟[1] 陈志丹[1] 周念[1] 周玲[1] 沈世强[2] 

机构地区：[1]襄樊市中心医院普外科,湖北441021 [2]武汉大学人民医院普外科

出　　处：《腹部外科》2009年第2期118-120,共3页Journal of Abdominal Surgery

摘　　要：目的探讨静脉血液稀释疗法和潘通对肢体缺血再灌注损伤(I/R)预防作用。方法复制家兔左下肢缺血/再灌注损伤模型,分别在阻断左下肢前、阻断4h灌注1h和再灌注24h采集假手术组、I/R组、潘通组、血液稀释组和联合治疗组静脉血,测定血浆丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(erythrocuprein,SOD)、血栓素B2(TXB2)含量,应用SAS8.1对实验数据进行均数间双因素方差分析,两两比较用SNK-q检验。结果潘通组、血液稀释组和联合治疗组血浆MDA、TXB2含量较I/R组显著降低,SOD显著升高,而联合治疗组与潘通组、血液稀释组相比也有显著性差异(P值均<0.05)。结论静脉血液稀释和潘通可有效缓解肢体缺血再灌注损伤,而联用静脉血液稀释和潘通可取得更好的效果,两者具有良好的协同效应。Objective To investigate the preventional effect Of hemodilution and pentomer on ischemic and reperfusion injury of limbs. Methods Rabbits were treated by ischemic and reperfusion（I/ R） of limbs, and peripheral blood was sampled from the rabbits of sham operation group, I/R group, pentomer group, hemodilution group, pentomer ＋ hemodilution group before ischemia and after reperfu- sion. The levels of MDA, SOD, and TXB2 were measured. Results As compared with I/R group, the levels of MDA, and TXB2 in pentomer, hemodilution, and pentomer ＋ hemodilution groups were significantly decreased, but the level of SOD was significantly increased（P〈0. 05）. As compared with pentomer and hemodilution groups, the levels of MDA, and TXB2 in pentomer ＋ hemodilution group were decreased significantly, but the level of SOD was significantly increased（P〈0. 05）. Conclusion Pentomer or hemodilution can relieve I/R injury of limbs, and simultaneous administration showed synergetic effects.

关 键 词：下肢 再灌注损伤 血液稀释 

分 类 号：R654.3[医药卫生—外科学] R457[医药卫生—临床医学]