2型糖尿病患者单核细胞TLR4表达及其与血浆TNF-α相关性研究  

Expression of TLR4 in monocytes and its correlation with TNF-α in patients with type 2 diabetes

作　　者：陈海英[1] 曲鹏[1] 丁彦春[1] 王虹艳[1] 牛楠[1] 徐丹 李蕾[1] 

机构地区：[1]大连医科大学附属第二医院心血管内科,大连116027 [2]大连市金州区第一人民医院

出　　处：《临床内科杂志》2009年第4期261-263,共3页Journal of Clinical Internal Medicine

基　　金：国家自然科学基金资助项目（30670836）

摘　　要：目的探讨2型糖尿病患者单核细胞TLR4表达的变化及其与血浆炎症因子TNF-α的关系。方法应用实时定量PCR及流式细胞仪检测22例2型糖尿病患者及20例健康对照者外周血单核细胞TLR4 mRNA表达及TLR4阳性单核细胞比率，应用ELISA法检测血浆TNF-α浓度。结果2型糖尿病患者外周血单核细胞TLR4 mRNA相对表达量及TLR4阳性单核细胞比率为3．05±0．56、（14．93±3．48）％明显高于对照组1．48±0．30、（7．79±1．63）％（P〈0．05），血浆TNF-α浓度为（49．29±7．09）ng／L，高于对照组（39．55±5．22）ng／L（P〈0．05），TLR4阳性单核细胞比率与血浆TNF-α浓度正相关（r=0．543，P〈0．05）。结论2型糖尿病患者单核细胞TLR4表达增高，单核细胞TLR4可能参与了2型糖尿病的炎症反应。Objective To investigate changes of toll-like receptor （TLR）4 expression in monocytes from type 2 diabetic patients and the relationship between TLR4 expression and the level of plasma inflammatory cytokine TNF-α. Methods The expression of TLR4 mRNA in monocytes and the percentage of TLR4 positive monocytes were assayed by real-time RT-PCR and flow cytometry. Plasma TNF-α concentration was measured by ELISA. Results The expression of TLR4 mRNA in monocytes,the percentage of TLR4 positive monocytes and plasma TNF-α concentration were higher in type 2 diabetic patients than those in control subjects （ P 〈 0.05 ）. In type 2 diabetic patients, the percentage of TLR4 positive monocytes was positively correlated with plasma TNF-α. Conclusion Monocytes TLR4 may participate in the inflammatory response of type 2 diabetes.

关 键 词：2型糖尿病 TLR4 炎症 动脉粥样硬化 

分 类 号：R587.1[医药卫生—内分泌]