ICP-MS法研究3nm TiO_2的小鼠体内分布  

ICP-MS analysis for TiO_2 distribution in mice injected with 3 nm TiO_2 particles

作　　者：殷吉磊[1,2] 李玉峰[3] 康成[3] 诸颖[1] 李玉兰[1] 李文新[1] 龚千锋[2] 黄庆[1] 李晴暖[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800 [2]江西中医学院,南昌330006 [3]中国科学院研究生院,北京100049

出　　处：《核技术》2009年第4期313-316,共4页Nuclear Techniques

基　　金：国家重大基础研究专项基金(2006CB705600);国家自然科学基金(10775169);上海市纳米专项(0752nm021)资助

摘　　要：采用溶胶-凝胶法合成粒径为3nm的TiO2颗粒,通过尾静脉注射到小鼠体内,在不同时间点剖取重要脏器,并用电感耦合等离子体-质谱法(ICP-MS)测量各个器官内TiO2的含量。结果显示,尾静脉注射进入小鼠体内的TiO2,很快随血流分布到全身各脏器,滞留在体内的TiO2主要集中在肺、肝、脾三脏,每克组织的摄取量(%ID/g)分别在6h、1h、1h达到最大值141.73%、25.55%、18.07%。实验还发现,对照组小鼠脑中Ti的含量高于血液,静脉注射3nmTiO2后,小鼠脑中Ti的含量显著增加,在实验的各个时间点(除0.5h),实验组小鼠脑中Ti的含量都比血液高。结果表明纳米TiO2颗粒的脑毒性研究可能成为其生物效应研究的重点。In this work, TiO2 nanoparticles of 3 nm in size were synthesized by sol-gel technique and injected intravenously into mice. The organs were collected at 0.5, 1, 3, 6, 24 and 48 h after injection. Ti content in the organs was detected by inductively coupled plasma mass spectrometry （ICP-MS）. The results indicated that injected TiO2 nanoparticles were translocated into the organs via bloodstream immediately after injection, and deposited mostly in lung, liver and spleen. The TiO2 uptake （%ID/g） in the three organs reached the peak value of 141.73%, 25.55%, and 18.07% at 6 h, 1 h and 1 h after injection, respectively. The results also showed that there was significant amount of Ti in the brains of the control, and the brain Ti content increased markedly after injection. At 1, 3, 6, 24 and 48 h after injection the brain Ti content was higher than that in blood. The experiment indicated that its toxicity on brain would be the emphasis of biological impact study of nano TiO2.

关 键 词：TIO2 静脉注射 ICP-MS 生物分布 

分 类 号：TB383[一般工业技术—材料科学与工程] O657.63[理学—分析化学]