钛表面自组装牛血清白蛋白以控制血小板粘附行为的研究  

Assembled bovine serum albumin onto titanium surface for improving the blood platelet adhesive behavior

作　　者：陈诚[1] 陈俊英[1] 李全利[1] 黄楠[1] 

机构地区：[1]西南交通大学材料科学与工程学院材料先进技术教育部重点实验室,四川成都610031

出　　处：《功能材料》2009年第4期660-662,666,共4页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(30670564);国家重点基础研究发展计划(973计划)资助项目(2005CB623904);教育部新世纪人才支持计划资助项目(06-0800)

摘　　要：将牛血清白蛋白通过自组装方法组装到钛表面,以改善其血液相容性。首先,用氢氧化钠活化钛,得到有活性羟基且带负电荷的多孔表面,然后浸入带正电荷的聚赖氨酸溶液,最后浸入带负电荷的牛血清白蛋白溶液。通过扫描电子显微镜(SEM)和原子力显微镜(AFM)观察自组装前后表面形貌变化,通过傅立叶红外漫反射(FTIR)检测各步处理层表面基团变化,通过视频接触角观察各步处理后钛表面接触角变化,通过体外血小板粘附实验评价自组装前后表面血液相容性变化。实验结果表明,牛血清白蛋白组装到钛表面后,血小板的粘附行为得到有效控制,钛表面的血液相容性显著改善。The bovine serum albumin was assembled onto the titanium surface by a layer-by-layer self-assembly （LBL） technique for improving the blood compatibility of titanium. Titanium was first treated by NaOH solution for obtaining negative charge and pores surface with activity. Then, the sample was immersed into poly-Llysine（PLL） solution for changing surface negative charge into positive charge. Finally, the sample was soaked in the bovine serum albumin （BSA） solution with negative charge for assembling BSA to titanium surface. The surface topography, the chemical composition and the wettability were investigated by scanning electron micro- scope （SEM）, atomic force microscope （AFM）, Fourier transform infrared spectroscopy （FTIR） and water contact angle measurement, respectively. The blood platelet adhesive behavior was evaluated by in vitro platelet adhesion experiment. The experiment result proved that the platelet adhesion had been well-controlled. The assembled titanium surface possesses the improving blood-compatibility.

关 键 词：钛 自组装 牛血清白蛋白 血小板 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程] R318.1[医药卫生—基础医学]