脂肪分化相关蛋白在巨噬细胞和泡沫细胞中的表达  

Expression profile of ADRP in macrophages and foam cells

作　　者：宋越[1] 李航[1] 张丽英[1] 叶菁[1] 李青[1] 

机构地区：[1]第四军医大学基础部病理学与病理生理学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2009年第4期309-311,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30671087)

摘　　要：目的:观察脂肪分化相关蛋白(ADRP)在单核细胞向巨噬细胞和泡沫细胞分化过程中的表达变化。方法:体外诱导THP-1单核细胞分化为巨噬细胞和泡沫细胞,应用RealtimeRT-PCR和Western blot测定ADRP在基因水平和蛋白水平的表达。结果:单核细胞分化为巨噬细胞时ADRP的表达水平无明显变化,当进一步分化为泡沫细胞时,ADRP的mR-NA和蛋白表达水平均显著增加。结论:单核细胞向巨噬细胞分化时对ADRP的表达无影响,而由巨噬细胞进一步向泡沫细胞分化时ADRP的表达升高。AIM： To investigate the expression profile of adipose differentiation-related protein （ADRP） in macrophages and foam cells. METHODS： THP-1 monocytes were differentiated into macrophages in vitro, and then induced into foam cells by culturing with oxidized low density lipoproteins（ox-LDLs）. Real time RT-PCR and Western blot were performed to detect the expression of ADRP gene and protein. RESULTS： The expression of ADRP both mRNA and protein were significantly increased when macrophages were further induced into foam cells by ox-LDLs, however, the same results were not observed during phorbol 12-myristate- 13-acetate（ PMA）-induced monocyte differentiation into macrophages. CONCLUSION： Our findings revealed an upregulation of ADRP during macrophages differentiation into foam cells.

关 键 词：脂肪分化相关蛋白 脂滴 巨噬细胞 泡沫细胞 动脉粥样硬化 

分 类 号：R392.11[医药卫生—免疫学]