他克莫司对UVA照射后HaCaT细胞增殖的影响  

The Inhibitory Effect of Tacrolimus on the Proliferation of Keratinocytes Irradiated by UVA

作　　者：付兰[1,2] 郑永唐[3] 张韡[1] 农祥[1] 涂颖[1] 郑博文[1] 何黎[1] 

机构地区：[1]昆明医学院第一附属医院皮肤科,云南昆明650032 [2]昆明市第一人民医院,云南昆明650011 [3]中国科学院昆明动物研究所,云南昆明650223

出　　处：《中国皮肤性病学杂志》2009年第4期202-204,共3页The Chinese Journal of Dermatovenereology

基　　金：云南省教育厅科学研究基金(编号:07Z11040)

摘　　要：目的探讨不同浓度他克莫司(TM)对不同强度长波紫外线(UVA)照射HaCaT细胞24h和48h后细胞增殖活性的影响。方法将培养的HaCaT细胞分别行2,4和8J/cm2的UVA照射,且照射前1h分别加入50,500和5000pg/mL的TM,对照组分别加入50,500和5000pg/mL的TM,但不行UVA照射。各剂量组分别照射24h和48h后在倒置相差显微镜下观察细胞的形态变化,MTT法检测细胞的增殖活性。结果HaCaT细胞经UVA照射24h后,细胞连接松散,细胞折光性较未照射组差,部分细胞死亡、脱壁;与未经UVA照射组相比,细胞增殖受到抑制(P<0.05),加入TM组无明显变化(P>0.05);照射48h后细胞虽有大量增殖,但体积较小;与未经UVA照射组相比,细胞增殖明显(P<0.05),加入TM组细胞增殖受到抑制(P<0.05)。结论TM可抑制UVA照射HaCaT细胞引起的过度增殖,对UVA照射后的HaCaT细胞有保护作用。Objective To investigate the effect of Tarcrolimus （TM） with different concentrations on the proliferation of HaCaT cell after UVA exposure with different intensity in 24h and in 48h. Methods All groups were pretreated by TM with different concentrations （50pg/mL, 500pg/mL and 5 000pg/mL, respectively）. In the experimental groups, the cultured HaCaT cells were exposed on different intensity of UVA （2J/cm^2, 4 J/cm^2 and 8J/cm^2, respectively） 1 hour before the pretreatment of TM, while no UVA exposure was used in the control groups. The morphological chang of HaCaT cells was observed by Contract Microscope in 24h and in 48h after UVA exposure. MTF assay was used to detect the proliferation of HaCaT cells. Results 24h after the exposure of UVA, the junction of HaCaT cells fell apart and the light refraction decreased compared with the control groups and some HaCaT cells were apoptosis and detached in the experimental groups. Moreover, the proliferation of HaCaT cells was inhibited in the groups exposed on UVA（ P 〉 0.05 ）and no influence was observed in the groups pretreated by TM（P 〉 0.05）. 48h after the exposure of UVA, the proliferation of HaCaT cells augmented but the volume of cells decreased. The proliferation augmented significantly in the groups with UVA exposure compared with that in the groups without UVA exposure（ P 〉0.05 ）. The proliferation was inhibited significantly in the groups pretreated by TM than that without TM pretreatment. Conclusion TM can inhibit the excessive proliferation of HaCaT cells after UVA exposure. It is protective for the HaCaT cell after the UVA exposure.

关 键 词：他克莫司 长波紫外线 HACAT细胞 

分 类 号：R286.0[医药卫生—中药学] R392.12[医药卫生—中医学]