基于大肠杆菌的CellSense生物传感器毒性分析性能研究  

Acute Toxicity Analysis Performance of CellSense Biosensor with E.coli

作　　者：王学江[1] 王虹[1] 赵建夫[1] 夏四清[1] 赵红宁[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

出　　处：《环境科学》2009年第4期1210-1214,共5页Environmental Science

基　　金：国家自然科学基金项目(20707014);教育部留学回国人员科研启动基金项目

摘　　要：采用聚碳酸酯膜直接固定法制备并优化了大肠杆菌(E.coli Top10)CellSense生物传感器微生物电极,探讨了其在重金属和有机污染物生物急性毒性分析中的应用性能.结果表明,基于对数生长后期和稳定期E.coli微生物电极的CellSense生物传感器具有良好的毒性分析性能,基于衰减期E.coli菌株的CellSense生物传感器毒性分析的稳定性和灵敏性降低;CellSense生物传感器测试得Hg2+、Cu2+、Zn2+、邻氯苯酚和对硝基苯酚对E.coli的EC50分别为0.6、3.1、5.81、80和94μg/mL,制备的E.coli微生物电极在冰箱中4℃保存2个月,仍能很好地满足毒性分析的需要.E. coli microbial electrodes for CellSense biosensor were prepared by polycarbonate membrane immobilization process, and their performance for heavy metals and toxic organic compounds acute toxicity determination were studied. The results showed that when E. coli was in logarithmic and stationary phase, the CellSense biosensor with E. coli showed good performance in heavy metal ions and organic pollutants acute toxicity analysis, when E. coli was in its decline phase, the stability and sensitivity of the CellSense biosensor was poor. The EC50 values of Hg^2＋ , Cu^2＋ , Zn^2＋ , o-chlorophenol （2-CP） and p-nitrophenol （4-NP） detected by CellSense biosensor with E. coli were 0.6, 3.1, 5.8, 180 and 94 μg/mL, respectively. The immobilized E. coli electrodes could still suit for acute toxicity assessment after 2 months storage at 4℃ .

关 键 词：微生物传感器 大肠杆菌 毒性 重金属 

分 类 号：X83[环境科学与工程—环境工程]