产气肠杆菌燃料电池产电机制研究  

Mechanisms of Bioelectricity Generation in Enterobacter Aerogenes-Based Microbial Fuel Cells

作　　者：张锦涛[1,2,3] 周顺桂[2] 张礼霞[2] 卢娜[2,3] 邓丽芳[2] 倪晋仁[1,3] 

机构地区：[1]北京大学深圳研究生院城市人居环境科学与技术重点实验室,深圳518055 [2]广东省生态环境与土壤研究所,广州510650 [3]北京大学环境工程系,北京100871

出　　处：《环境科学》2009年第4期1215-1220,共6页Environmental Science

基　　金：国家自然科学基金项目(20777013);广东省自然科学基金项目(07006759)

摘　　要：基于铁还原菌的微生物燃料电池(MFCs)与基于产氢细菌的MFCs各有优劣,前者能量利用效率高,但底物利用范围有限;后者底物利用广泛,但其依靠悬浮细胞产电,电子回收率较低.本研究以1株具有铁还原活性的产氢菌Enterobacter aerogenes XM02为产电微生物,构建了3种不同阳极材料的空气阴极MFCs,通过输出电压和库仑效率的比较以及扫描电镜观察阳极形貌等方法对此菌的产电机制进行探讨.结果发现,采用比表面积大且具有氢催化活性的碳毡作为阳极材料时,可显著改善MFCs性能,库仑效率由载铂碳纸阳极MFC的1.68%提高至42.49%,远高于已报道的其它产氢菌燃料电池.扫描电镜证实大量细菌细胞附着在阳极表面,形成阳极生物膜.采用非生长基质实验排除悬浮细胞的产电作用,证明附着在阳极的生物膜对产电起主导作用,认为生物膜原位产氢-氧化是此菌的主要产电机制.Microbial fuel cells （MFCs） using hydrogen-producing bacteria （HPB） could utilize a large number of substrates to generate power. However, the coulombic efficiency is limited by the fact that only suspended cells are used as biocatalyst in anodic medium. MFCs using Fe （Ⅲ）-reducing bacteria have high energy recovery efficiency, but can only utilize some simple organic matters. In this study, Enterobacter aerogenes XM02, a hydrogen-producing strain with Fe （Ⅱ）-reducing activity, was selected as biocatalyst for MFCs, which could produce electricity by digesting lots of carbohydrates even starch. Graphite felt, a material with high specific surface area and hydrogen catalysis, instead of carbon paper supported platinum, was used as anode material. The coulombic efficiency had been substantially improved from 1.68 % to 42.49% , higher than other HPB-based MFCs previously reported. The SEM image proved the ability of XM02 strain to colonize on the anode surface. Power generation of MFCs could restore quickly when anodic medium was completely replaced with non-growth medium containing glucose. This suggested that the attached cells contributed to electricity production because planktonic cells had been removed during the medium replacement. This study proposed the mechanism of power generated from in situ oxidation of hydrogen produced by the XM02 strain biofilm.

关 键 词：微生物燃料电池 产气肠杆菌 库仑效率 电子回收 生物膜 

分 类 号：X382[环境科学与工程—环境工程]