内皮素-1基因多态性与原发性高血压的相关性研究  

Relationship between endothelin-1 gene polymorphisms and essential hypertension

作　　者：杭丽玮[1] 吴志红[2] 王珍[1] 窦贵旺[2] 蔡家春[2] 周建中[1] 

机构地区：[1]重庆医科大学:附属第一医院心血管内科,重庆400016 [2]重庆医科大学公共卫生学院流行病学教研室,重庆400016

出　　处：《第三军医大学学报》2009年第8期684-688,共5页Journal of Third Military Medical University

基　　金：重庆市科委自然科学基金(2006BB5044);重庆医科大学校办课题(QD200535)~~

摘　　要：目的探讨重庆地区人群血管内皮素-1(endothelin-1,ET-1)基因Lys198Asn、TaqⅠ酶切位点基因多态性与原发性高血压(essential hypertension,EH)的关系。方法运用序列特异引物聚合酶链反应(sequence specific primer poly-merase chain reaction,SSP-PCR)和限制性内切酶片段长度多态性(restriction fragment length polymorphism,RFLP)方法分析86例EH患者和138例非EH患者的Lys198Asn和TaqⅠ2个位点基因多态性。结果体质量指数(body mass index,BMI)<25 kg/m2人群中高血压组的TaqⅠ酶切位点基因型分布频率为:GG型26例(72.2%),GA+AA型10例(27.8%);对照组:GG型28例(50.0%),GA+AA型28例(50.0%)。Logistic回归分析显示BMI<25 kg/m2人群中携带GG基因型(TaqⅠ酶切位点)的患者发生高血压的风险增加(P=0.037,OR=2.597)。线性回归结果显示收缩压(SBP)在BMI<25 kg/m2人群的GG型平均值为145.06,显著高于GA+AA型平均值131.97(P=0.018)。结论在BMI<25 kg/m2人群中TaqⅠ酶切位点GG基因型更易导致SBP升高,GG基因型可能是BMI较小人群血压升高的一个危险因素。Objective To investigate the relationship between Lys198Asn and Taq I polymorphisms of endothelin-1 gene and essential hypertension in Chongqing residents. Methods Polymorphisms of Lys198Asn and Taq I were detected in the blood samples fiom 86 patients with essential hypertension （HT） and 138 nor- motensive controls （NT） by sequence specific primer-polymerase chain reaction （SSP-PCR） and PCR-restfie- tion fragment length polymorphism （PCfI-RFLP） Results In the BMI 〈25 kD/m2 subjects, the frequency of GG and CA ＋ AA of Taq I was 26 （72.2% and 10 （27. 8% ） respectively in hypertensive group, 28 （50. 0% ） and 28 （50.0%） respectively in normotensive group. Logistic regression model analysis showed that the GG carriers of Taq I had a significant high risk to develop hypertension （P = 0. 037, OR = 2. 597） in BMI 〈 25 kg/m2 subjects. The liner regression model analysis demonstrated that there was a significant difference in systolic blood pressure （SBP） between CG and CA ＋ AA in BMI 〈 25 kg/m2 subjects （P = O. 018）. SBP of the GG carriers （145.06 mmHg） were 13.09 mmHg higher than those of CA ＋ AA carriers （131.97 mmHg）. Conclusion Among the BMI 〈25 kg/m2 subjects, those with GG carriers of Taq I might be prone to have higher SBP. The GG genotype might be a risk factor for hypertension in normoweight subjects.

关 键 词：内皮素-1 基因多态性 原发性高血压 

分 类 号：R181.33[医药卫生—流行病学] R394.3[医药卫生—公共卫生与预防医学]