五倍子小鼠肝脏毒性的基因表达谱分析(英文)  

GENE EXPRESSION PROFILING OF MICE LIVER TISSUE AFTER INTRAGASTRIC ADMINISTRATION OF CHINESE NUTGALL EXTRACT

作　　者：韩凤梅[1] 张晓鸣[1] 夏启松[1] 王俊俊[1] 陈勇[1] 

机构地区：[1]湖北大学中药生物技术省重点实验室,武汉430062

出　　处：《分子细胞生物学报》2009年第2期101-108,共8页Journal of Molecular Cell Biology

基　　金：supported by Prophase Special Funds for Major State Basic Research of China(No. 2002ccc00300)

摘　　要：昆明种小鼠(雄性,体重20±2g)随机分为正常对照组和五倍子给药组。给药组每日灌胃五倍子提取物(0.2mL/10g体重,相当于8g五倍子生药/1kg体重)一次,连续给药30天。对照组灌胃等量生理盐水。之后分别提取两组小鼠的肝组织总mRNA,经反转录分别用Cy3,Cy5荧光标记,制备用于芯片杂交的cDNA探针。两种探针等量混合后与小鼠全基因组寡核苷酸芯片杂交,杂交信号经芯片扫描仪获取并用GenePix Pro4.0软件分析。结果表明,五倍子给药组中有461条基因差异表达,其中上调基因267条,下调基因194条,功能已知基因373条,功能未知基因88条。通过对这些差异表达基因的生物学功能及生物通路分析,它们主要涉及代谢、DNA结合与转录、蛋白质合成与修饰、细胞骨架及黏附因子、细胞周期与分化、离子通道与受体、信号转导、免疫、细胞凋亡等。上述研究结果对分析五倍子肝损伤机制可能具有十分重要的作用。Kunming mice （male, weight 20 ± 2g） were daily intragastric administration of Chinese nutgall extract （0.2 mL/10g body weight, equal to 8g Chinese nutgall material/1 kg body weight） for 30 days. Liver tissue mRNA were extracted from normal control group and Chinese nutgall treated group respectively, then were reversely transcribed to cDNA with dUTP labeled by different fluorescence （Cy3, Cy5） as hybridization probes. Both of the cDNA probes were mixed equally in 20μL of hybridization solution and hybridized with mice complete genome oligonucleotide microarray. The fluorescent signals were acquired by laser scanner and analyzed by GenePix Pro 4.0 software. The biological function analysis and the pathway analysis of differentially expressed genes were performed according to Gene Ontology database. As a result, there were 461 genes differentially expressed in Chinese nutgall treated group, in which 373 genes were function-known and the others were function-unknown. Among the 461 genes, 267 genes were up-regulated and the others were down-regulated. The differentially expressed genes were involved in metabolism, DNA binding and transcription, protein synthesis and modification, cell cytoskeleton and cell adhesion, cell cycle and differentiation, ion channels and transporters, signal transduction, immune responseand apoptosis of liver cell. The presented work might be quite important for understanding the pathogenic mechanisms of liver injury induced by Chinese nutgall.

关 键 词：五倍子 肝损伤 基因表达谱 寡核苷酸芯片 

分 类 号：R285.5[医药卫生—中药学]