白介素17在实验性支气管哮喘小鼠气道杯状细胞化生和黏液分泌中的作用  

Effects of interleukino-17 on metaplasia of goblet cell and secretion of Muc5ac protein in airway of experimental bronchial asthma mouse

作　　者：魏然[1] 刘剑波[1] 

机构地区：[1]郑州大学第二附属医院呼吸内科,450014

出　　处：《国际呼吸杂志》2009年第7期399-401,共3页International Journal of Respiration

基　　金：河南省自然科学基金项目（511044300）

摘　　要：目的观察经鼻滴入白介素17（interleukin-17，IL-17）对支气管哮喘（简称哮喘）小鼠气道杯状细胞表达Muc5ac黏蛋白的影响，探讨IL-17与哮喘黏液分泌的关系。方法应用免疫组织化学法测定正常对照组、哮喘组、IL-17组及抗IL-17组肺组织黏蛋白Muc5ac的表达及应用AB-PAS染色观察支气管壁杯状细胞数量的差异。结果Muc5ac蛋白阳性表达细胞均主要位于气道上皮，细胞胞浆呈棕黄色。与哮喘组相比，IL-17组黏蛋白Muc5ac表达量明显增加（P〈0．01），抗IL-17组表达显著减少（P〈0．01）。AB-PAS染色结果同上。结论IL-17具有增强哮喘小鼠气道黏液分泌的作用。Objective To investigate the effect of administering intranasally interleukin-17（IL-17） on goblet cell metaplasia of bronchial asthma（asthma） mouse airway and the secretion of Muc5ac protein. Methods Forty female BALB/c mice were randomly divided into normal control group,asthma group,IL-17 group, anti-IL-17 group, ten mice per group. Muc5ac protein expressions were detected by immunohistoehemical stain for the numbers of goblet cells were differented by the AB-PAS stain. Results Muc5ac positive products were located in the cytoplasm of airway epithelial cells. Compared with that of control group, IL-17 increased Muc5ac protein expressions （P〈0. 01）, while anti-IL-17 group obviously decreased Muc5ac protein expressions （ P〈0.01）. AB-PAS stain had the same result with Muc5ac protein expressions. Conclusions IL-17 has the ability of enhancing secretion of Muc5ac protein.

关 键 词：哮喘 小鼠 白介素17 黏液 杯状细胞 

分 类 号：R5[医药卫生—内科学]