外周血中单个网织红细胞与小淋巴细胞的拉曼光谱  

Raman Spectra of Single Reticulocytes and Small Lymphocytes in Blood

作　　者：艾敏[1,2] 刘军贤[2] 姚辉璐 

机构地区：[1]广西科学院生物物理实验室,广西南宁541004 [2]广西师范大学物理与电子工程学院,广西桂林541004

出　　处：《光学学报》2009年第4期1043-1048,共6页Acta Optica Sinica

基　　金：国家自然科学基金(60868002);广西科学基金(0575027;0718007b-30)项目

摘　　要：为了在外周血中准确区分大小相近的网织红细胞和小淋巴细胞,采用光镊与拉曼光谱结合的单细胞技术。研究发现大鼠血液中单个网织红细胞和小淋巴细胞的光谱形态相差很大,主要体现在以下位置:1212,1548,1607,1616,1001,1661 cm^(-1)(以上与蛋白质相关),1584 cm^(-1)(与核酸相关)。通过分析部分特征峰,证明小淋巴细胞相对于网织红细胞而言,所含有的核酸含量高,蛋白质含量少。通过主成分分析和判别分析两种统计方法进行分析,均可完全区分这两种细胞。通过上述分析,得到了一种光谱学上快速、准确地区分外周血中网织红细胞和小淋巴细胞的方法,同时也为在现代生物医学研究血细胞提供一种识别依据和手段。The single-cell-molecular technique based on optical tweezers and Raman spectroscopy is employed to study single reticulocytes and small lymphocytes of Sprague Dawley （S. D. ） rat in nearly physiological solution. The spectra of single reticulocytes and small lymphocytes are obviously different due to their components. The main differences show that characteristic Raman bands of the protein are 1212, 1548, 1607 and 1616 cm^-1 for reticulocytes, 1001 and 1661 cm^-1 for small lymphocytes, and that of nucleic acid is 1584 cm^-1 for both of them. The content of nucleic acid of small lymphocytes is more than that of reticulocytes, but for protein it is reverse by the histogram analysis of part characteristic Raman bands. Furthermore, the reticulocytes and small lymphocytes can be distinguished completely by principal component analysis and discriminant function analysis. The way of distinguishing quickly and accurately of single reticulocytes and small lymphocytes by the Raman spectra cannot be realized by optical microscope. It also provides a new method to distinguish two kinds of cells in blood for modern biomedicine study.

关 键 词：光谱学 区分网织红细胞与白细胞 拉曼光谱分析 光镊 单细胞 

分 类 号：O436[机械工程—光学工程]