糖尿病及并发症血清的表面增强拉曼光谱  

Surface-Enhanced Raman Spectra Analysis of Serum from Diabetes Mellitus and Complication

作　　者：韩洪文[1] 闫循领[1] 班戈[1] 李珂[1] 杨颖[2] 邴艳霞[2] 

机构地区：[1]聊城大学物理科学与信息工程学院,山东聊城252059 [2]聊城市人民医院,山东聊城252000

出　　处：《光学学报》2009年第4期1122-1125,共4页Acta Optica Sinica

基　　金：国家自然科学基金(60571062);山东省自然科学基金(Y2004G09)资助

摘　　要：为了从分子水平上探讨病人血清中组织、细胞代谢产物的生化异常,利用表面增强拉曼散射技术检测了糖尿病及并发症(冠心病、青光眼和脑梗塞)病人的血清。结果表明:这几种疾病患者血清的表面增强拉曼光谱十分相似,说明这几种疾病中组织、细胞代谢产物的分子结构或含量变化相近;与正常人血清的表面增强拉曼光谱相比,存在显著的差异。特别是在这几种疾病患者血清中,谱线725 cm^(-1)相对强度明显增强,且增强部分来自腺嘌呤的贡献,与血糖的高低无关,说明这几种疾病患者的代谢产物中含有较高的碱基腺嘌呤,这些结果为研究糖尿病及并发症的生化机理以及临床诊断提供有力的实验依据。In order to study the biochemical abnormality of metabolites from tissues or cells at the molecular level, the surface enhanced Raman scattering technique was used to detect the serums from diabetes mellitus and complication （coronary disease, glaucoma and cerebral infarction）. It is found that the characteristics surfaceenhanced raman spectra （SERS） of serums are very similar to that from diabetes mellitus, which indicates that the changes of molecular structure or content from the metabolites of tissues or cells in these diseases are similar. The significant differences of SERS are found between normal and diseased serums. Especially, the relative intensity of the band from patients increases obviously at 725 cm-~. The partial reinforcement comes form the adenines＇ contribution and is independent of the blood sugar level, which indicates that there exist many adenines in these serums. The results will offer the experimental basis for the biochemical study and clinical diagnosis of diabetes mellitus and complication.

关 键 词：光谱学 代谢产物的生化异常 表面增强拉曼光谱 糖尿病及并发症 血清 腺嘌呤 

分 类 号：O657.37[理学—分析化学]