小儿急性阑尾炎细菌移位及IL-10、NO水平变化的临床意义  

Study on clinical value of bacterial translocation and expression change of NO and IL-10 in acute appendicitis in children

作　　者：崔娆[1] 王建峰[1] 

机构地区：[1]山西医科大学儿科系,太原030001

出　　处：《中国现代药物应用》2009年第8期1-3,共3页Chinese Journal of Modern Drug Application

基　　金：山西省科技攻关项目(项目编号:20080311064-2)

摘　　要：目的探讨小儿急性阑尾炎细菌移位、一氧化氮(NO)、白介素-10(IL-10)的变化及临床意义。方法应用聚合酶链反应(PCR)定性检测阑尾炎组及对照组全血中的细菌基因;应用酶联免疫吸附试验(ELISA)定量检测阑尾炎组术前、术后7d和对照组血清中NO、IL-10的水平。结果急性单纯性阑尾炎组16SrRNA、大肠杆菌(BG)未检出;化脓性阑尾炎组及坏疽性阑尾炎组16SrRNA阳性率分别为70%、80%,BG阳性率分别为60%、70%;阑尾炎术前组NO浓度与术后组差异有统计学意义(P<0.01);阑尾炎术前组NO浓度与对照组间差异有统计学意义(P<0.01);术后组差异无统计学意义(P>0.05);阑尾炎术前组IL-10浓度与术后组间比较差异有统计学意义(P<0.01);阑尾炎术前组IL-10浓度与对照组间比较差异有统计学意义(P<0.01)。结论小儿急性阑尾炎细菌移位的发生,NO、IL-10起着重要作用,检测其变化对细菌移位的发生机制及临床诊治有一定的价值。Objective To,evaluate the acute appendicitis in children with bacterial translocation occurred , appendicitis in children pre-operation and after-operation 7 days of serum NO （nitric oxide） and IL-10 （interleukin 10） ; Methods PCR （polymerase chain reaction） testing as appendicitis and control groups of bacteria in whole blood; Application of ELISA （ enzyme-linked immunosorbent assay） quantitative detection of appendicitis group pre-operation and after-operation 7 days and the control group serum levels of NO and IL-10; Result The control group and simple acute appendicitis group in whole blood were not detected bacteria; Purulent appendicitis group and gangrenous appendicitis group 16SrRNA positive rates were 70%, 80%, BG positive Rates were 60%, 70% ; Appendicitis pre-operative group, the concentration of NO was different from the after-operative group, statistically significant （P 〈 0. 01 ） ;Appendicitis pre-operative group, the concentration of NO was different from the control group, statistically significant （ P 〈 0. 01 ） ; After operative group had no difference, not significant （P 〉 0. 05 ） ; Appendicitis pre-operative group, the concentration of IL-10 was different from the after-operative group, statistically significant （P 〈 0. 01 ） ; Appendicitis pre-operative group , the concentration of IL-10 was different from the control group ,statistically significant （P 〈0. 01 ） ;After operative group had no difference , not significant （ P 〉 0. 05） ; Conclusion Acute appendicitis in children incides bacterial translocation, detection of NO and IL-10 in children with acute appendicitis play an important role in the mechanism of bacterial translocation and a certain clinical value.

关 键 词：小儿急性阑尾炎 细菌移位 一氧化氮 白介素-10 

分 类 号：R726.5[医药卫生—儿科]